Overview

  • Product nameAnti-Cytochrome p450 2J2 antibody
    See all Cytochrome p450 2J2 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome p450 2J2
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) derived from an internal region of Cytochrome p450 2J2.

  • Positive control
    • Extracts from A549, RAW264.7 and NIH-3T3 cells. Human heart tissue. HepG2 and COS7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab69651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IHC-P
ICC/IF
  • Application notesELISA: 1/10000.
    ICC/IF: 1/500 - 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). Whole Cell Lysis Buffer (4?) is recommended. For example: Solution Final Concentration
    KCl 50mM
    NP-40 1%
    HEPES (pH7.8) 25mM
    DTT 125uM
    Na3VO4 1mM
    NaF 1mM
    + fresh protease and phosphatase inhibitors

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
    • Tissue specificityHighly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.
    • Sequence similaritiesBelongs to the cytochrome P450 family.
    • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Arachidonic acid epoxygenase antibody
      • CP2J2_HUMAN antibody
      • CPJ2 antibody
      • CYP2J2 antibody
      • CYPIIJ2 antibody
      • Cytochrome P450 2J2 antibody
      • Cytochrome P450 family 2 subfamily J polypeptide 2 antibody
      • Cytochrome P450 subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2 antibody
      • Cytochrome P450 subfamily IIJ polypeptide 2 antibody
      • Flavoprotein linked monooxygenase antibody
      • Microsomal monooxygenase antibody
      • OTTHUMP00000010550 antibody
      see all

    Anti-Cytochrome p450 2J2 antibody images

    • All lanes : Anti-Cytochrome p450 2J2 antibody (ab69651) at 1/500 dilution

      Lane 1 : Extracts from A549 cells
      Lane 2 : Extracts from RAW264.7 cells
      Lane 3 : Extracts from NIH-3T3 cells
      Lane 4 : Extracts from NIH-3T3 cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 58 kDa
      Observed band size : 58 kDa
    • Immunohistochemistry analysis of paraffin-embedded human heart tissue stained with ab69651 at 1/50 dilution, with and without the immunising peptide.
    • Immunofluorescence analysis of HepG2 cells using ab69651 at 1/500 dilution, with and without the immunising peptide.

    References for Anti-Cytochrome p450 2J2 antibody (ab69651)

    This product has been referenced in:
    • Bystrom J  et al. Inducible CYP2J2 and Its Product 11,12-EET Promotes Bacterial Phagocytosis: A Role for CYP2J2 Deficiency in the Pathogenesis of Crohn's Disease? PLoS One 8:e75107 (2013). Read more (PubMed: 24058654) »

    See 1 Publication for this product

    Product Wall

    Thank you for your reply and for providing the additional information.

    You may want to try loading a whole cell lysate as this was what was tested in our lab. It may be that there is not enough protein present in the microsomal prep alone to ...

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    Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

    Did you place this order through Migliore? I see an order from April 2011, so unfortunately this antibody is over a year ol...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"