Anti-Cytochrome p450 2J2 antibody (ab76176)

Overview

  • Product name
    Anti-Cytochrome p450 2J2 antibody
    See all Cytochrome p450 2J2 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome p450 2J2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Cytochrome p450 2J2.

    (Peptide available as ab88195.)

  • Positive control
    • This antibody gave a positive signal in HEK293 whole cell lysate and in the following tissue lysates: Human heart; Human liver; Human kidney; Human colon.

Properties

Applications

Our Abpromise guarantee covers the use of ab76176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Target

  • Function
    This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
  • Tissue specificity
    Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Arachidonic acid epoxygenase antibody
    • CP2J2_HUMAN antibody
    • CPJ2 antibody
    • CYP2J2 antibody
    • CYPIIJ2 antibody
    • Cytochrome P450 2J2 antibody
    • Cytochrome P450 family 2 subfamily J polypeptide 2 antibody
    • Cytochrome P450 subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2 antibody
    • Cytochrome P450 subfamily IIJ polypeptide 2 antibody
    • Flavoprotein linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000010550 antibody
    see all

Images

  • All lanes : Anti-Cytochrome p450 2J2 antibody (ab76176) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

References

ab76176 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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