Anti-Cytochrome p450 2J2 antibody (ab76176)

Overview

  • Product nameAnti-Cytochrome p450 2J2 antibody
    See all Cytochrome p450 2J2 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome p450 2J2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Cytochrome p450 2J2.

    (Peptide available as ab88195.)

  • Positive control
    • This antibody gave a positive signal in HEK293 whole cell lysate and in the following tissue lysates: Human heart; Human liver; Human kidney; Human colon.

Properties

Applications

Our Abpromise guarantee covers the use of ab76176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Target

  • FunctionThis enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
  • Tissue specificityHighly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Arachidonic acid epoxygenase antibody
    • CP2J2_HUMAN antibody
    • CPJ2 antibody
    • CYP2J2 antibody
    • CYPIIJ2 antibody
    • Cytochrome P450 2J2 antibody
    • Cytochrome P450 family 2 subfamily J polypeptide 2 antibody
    • Cytochrome P450 subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2 antibody
    • Cytochrome P450 subfamily IIJ polypeptide 2 antibody
    • Flavoprotein linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000010550 antibody
    see all

Anti-Cytochrome p450 2J2 antibody images

  • All lanes : Anti-Cytochrome p450 2J2 antibody (ab76176) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

References for Anti-Cytochrome p450 2J2 antibody (ab76176)

ab76176 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab76176.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"