The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.
Western blot - Cytochrome p450 2J2 antibody (ab76176)
All lanes : Anti-Cytochrome p450 2J2 antibody (ab76176) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431) Lane 2 : Human liver tissue lysate - total protein (ab29889) Lane 3 : Human kidney tissue lysate - total protein (ab30203) Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 57 kDa Observed band size : 57 kDa Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.