Anti-Cytochrome P450 3A1 antibody (ab22733)


  • Product nameAnti-Cytochrome P450 3A1 antibody
    See all Cytochrome P450 3A1 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 3A1
  • Tested applicationsSuitable for: WB, IHC-FoFr, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, taken from the C-terminus of rat Cytochrome P450 3A1.

  • Positive control
    • PCN stimulated rat liver and microsomal preparations of liver.



Our Abpromise guarantee covers the use of ab22733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-F: Use at a dilution of up to 1/2000.
    IHC-Fr: Use at a dilution of up to 1/2000.
    WB: Use at an assay dependent dilution. Detects a band of approximately 58 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceIn animals, P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. Cytochrome P450 3A4 [human-3A1 Rat] is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
    • Cellular localizationEndoplasmic reticulum, membrane bound.
    • Database links
    • Alternative names
      • CYP3A1 antibody
      • cytochrome P450, family 3, subfamily a, polypeptide 1 antibody

    Anti-Cytochrome P450 3A1 antibody images

    • ab22733 staining Cytochrome P450 3A1 in rat liver tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone. Samples were then blocked with 2% serum for 1 hour at 20°C followed by incubation with the primary antibody, at a 1/500 dilution, for 1 hour at 20°C. An Atto 550 dye-conjugated goat anti-rabbit IgG polyclonal was used as secondary antibody at a 1/500.

      See Abreview

    References for Anti-Cytochrome P450 3A1 antibody (ab22733)

    This product has been referenced in:
    • Dublineau I  et al. Unexpected lack of deleterious effects of uranium on physiological systems following a chronic oral intake in adult rat. Biomed Res Int 2014:181989 (2014). WB ; Rat . Read more (PubMed: 24693537) »
    • Hassan HE  et al. Induction of xenobiotic receptors, transporters, and drug metabolizing enzymes by oxycodone. Drug Metab Dispos 41:1060-9 (2013). Rat . Read more (PubMed: 23439660) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us and I am sorry for the delay in getting back to you.

    I am pleased to hear that the replacement antibody provided has been working well for you. As I mentioned in my previous email, the homology of the CYP3A1 and CYP...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Liver)
    Specification Liver
    Fixative Acetone 10' at 4°C
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Oct 12 2010

    I can confirm that the antibodies were raised to a synthetic hexapeptide which includes the C-terminal of rat CYP3A110: H-Cys-Ile-Ile-Thr-Gly-Ser-OH. Both antibodies work equally well in western blotting and we cannot recommend one over the other, w...

    Read More