Anti-Cytochrome P450 3A1 antibody [P6] (ab22724)

Overview

  • Product nameAnti-Cytochrome P450 3A1 antibody [P6]
    See all Cytochrome P450 3A1 primary antibodies
  • Description
    Mouse monoclonal [P6] to Cytochrome P450 3A1
  • Tested applicationsSuitable for: Flow Cyt, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Full length protein (Rat). Rat liver cytochrome P450 3A1.

Properties

Applications

Our Abpromise guarantee covers the use of ab22724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.

Target

  • RelevanceIn animals, P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. Cytochrome P450 3A4 [human-3A1 Rat] is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
  • Cellular localizationEndoplasmic reticulum, membrane bound.
  • Database links
  • Alternative names
    • CYP3A1 antibody
    • cytochrome P450, family 3, subfamily a, polypeptide 1 antibody

Anti-Cytochrome P450 3A1 antibody [P6] images

  • Overlay histogram showing HepG2 cells stained with ab22724 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22724, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Cytochrome P450 3A1 antibody [P6] (ab22724)

This product has been referenced in:
  • Probert PM  et al. Utility of B-13 progenitor-derived hepatocytes in hepatotoxicity and genotoxicity studies. Toxicol Sci 137:350-70 (2014). WB ; Human . Read more (PubMed: 24235770) »
  • Hu N  et al. Opposite effect of diabetes mellitus induced by streptozotocin on oral and intravenous pharmacokinetics of verapamil in rats. Drug Metab Dispos 39:419-25 (2011). WB ; Rat . Read more (PubMed: 21135265) »

See all 3 Publications for this product

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The product ab22724 has been tested with rat samples so it is fully guaranteed. Please check the Abcam abpromise guarantee policy; http://www.abcam.com/index.html?pageconfig=abpromise

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I can confirm that ab22724 Cytochrome P450 3A1 antibody [P6] has been tested for cross-reactivity against other CYP3A family members but no evidence was seen to show that it might recognize CYP3A2. I am sorry this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - other (liver microsomal protein)
Loading amount 20 µg
Specification liver microsomal protein
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mrs. flower little

Verified customer

Submitted Jun 15 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (liver)
Loading amount 20 µg
Specification liver
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mrs. flower little

Verified customer

Submitted Jun 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"