Anti-Cytochrome P450 3A4 antibody (ab94334)

Overview

  • Product nameAnti-Cytochrome P450 3A4 antibody
    See all Cytochrome P450 3A4 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 3A4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus Monkey, Macaque Monkey, Marmoset (common), Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Cytochrome P450 3A4 .

  • Positive control
    • This antibody gave a positive signal in Human Liver Tissue Lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab94334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).

Target

  • FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
  • Tissue specificityExpressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Post-translational
    modifications
    Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1,8-cineole 2-exo-monooxygenase antibody
    • Albendazole monooxygenase antibody
    • Albendazole sulfoxidase antibody
    • CP33 antibody
    • CP34 antibody
    • CP3A4_HUMAN antibody
    • CYP3 antibody
    • CYP3A antibody
    • CYP3A3 antibody
    • CYP3A4 antibody
    • CYPIIIA3 antibody
    • CYPIIIA4 antibody
    • Cytochrome P450 3A3 antibody
    • Cytochrome P450 3A4 antibody
    • Cytochrome P450 family 3 subfamily A polypeptide 4 antibody
    • Cytochrome P450 HLp antibody
    • Cytochrome P450 NF-25 antibody
    • Cytochrome P450 subfamily IIIA polypeptide 4 antibody
    • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3 antibody
    • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 antibody
    • Cytochrome P450-PCN1 antibody
    • Glucocorticoid inducible P450 antibody
    • HLP antibody
    • MGC126680 antibody
    • NF 25 antibody
    • NF25 antibody
    • Nifedipine oxidase antibody
    • P450 III steroid inducible antibody
    • P450 PCN1 antibody
    • P450, family III antibody
    • P450C3 antibody
    • P450PCN1 antibody
    • Quinine 3 monooxygenase antibody
    • Quinine 3-monooxygenase antibody
    • Taurochenodeoxycholate 6 alpha hydroxylase antibody
    • Taurochenodeoxycholate 6-alpha-hydroxylase antibody
    see all

Anti-Cytochrome P450 3A4 antibody images

  • Anti-Cytochrome P450 3A4 antibody (ab94334) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • Anti-Cytochrome P450 3A4 antibody (ab94334) at 1 µg/ml + Recombinant Human Cytochrome P450 3A4 protein (ab114327) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

References for Anti-Cytochrome P450 3A4 antibody (ab94334)

ab94334 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab94334.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"