Anti-Cytochrome P450 3A5 antibody [F18 P3 B6] (ab56076)

Overview

  • Product name
    Anti-Cytochrome P450 3A5 antibody [F18 P3 B6]
    See all Cytochrome P450 3A5 primary antibodies
  • Description
    Mouse monoclonal [F18 P3 B6] to Cytochrome P450 3A5
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the C terminus of human Cytochrome P450 3A5

  • Positive control
    • Oesophageal Squamous cell carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab56076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aryl hydrocarbon hydroxylase antibody
    • CP35 antibody
    • CP3A5_HUMAN antibody
    • CYP3A5 antibody
    • CYPIIIA5 antibody
    • Cytochrome P450 3A5 antibody
    • Cytochrome P450 antibody
    • Cytochrome P450 family 3 subfamily A polypeptide 5 antibody
    • Cytochrome P450 HLp2 antibody
    • Cytochrome P450 subfamily IIIA (niphedipine oxidase) polypeptide 5 antibody
    • Cytochrome P450-PCN3 antibody
    • DKFZp686L16231 antibody
    • Flavoprotein linked monooxygenase antibody
    • FLJ31317 antibody
    • HLp2 antibody
    • Microsomal monooxygenase antibody
    • Niphedipine oxidase antibody
    • P450PCN3 antibody
    • PCN3 antibody
    • Xenobiotic monooxygenase antibody
    see all

Images

  • All lanes : Anti-Cytochrome P450 3A5 antibody [F18 P3 B6] (ab56076) at 2 µg/ml

    Lane 1 : CYP3A4 recombinant CYP450 protein
    Lane 2 : CYP3A5 recombinant CYP450 protein
    Lane 3 : CYP3A7 recombinant CYP450 protein


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • Lane 1 : Molecular weight markers
    Lanes 2 - 10 : Anti-Cytochrome P450 3A5 antibody [F18 P3 B6] (ab56076) at 2 µg/ml

    Lane 1 : As above
    Lane 2 : CYP1A1 recombinant protein
    Lane 3 : CYP2C18 recombinant protein
    Lane 4 : CYP2C19 recombinant protein
    Lane 5 : CYP2D6 recombinant protein
    Lane 6 : CYP4F2 recombinant protein
    Lane 7 : CYP4F3 recombinant protein
    Lane 8 : CYP3A5 recombinant protein
    Lane 9 : CYP3A7 recombinant protein
    Lane 10 : Negative control


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • Oesophageal squamous cell carcinoma tissue stained with ab56076 at 5 µg/ml.

References

ab56076 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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