The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Use a concentration of 1 - 5 µg/ml.
FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Sequence similaritiesBelongs to the cytochrome P450 family.
Lane 1 : As above Lane 2 : CYP1A1 recombinant protein Lane 3 : CYP2C18 recombinant protein Lane 4 : CYP2C19 recombinant protein Lane 5 : CYP2D6 recombinant protein Lane 6 : CYP4F2 recombinant protein Lane 7 : CYP4F3 recombinant protein Lane 8 : CYP3A5 recombinant protein Lane 9 : CYP3A7 recombinant protein Lane 10 : Negative control
Predicted band size : 57 kDa Observed band size : 57 kDa