Overview

  • Product nameAnti-Cytochrome P450 4A antibody
    See all Cytochrome P450 4A primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 4A
  • SpecificityThe immunogen is homologous to rat cytochrome P450 4A2, A10, A12 and A14. The specificity to these specific forms has not been confirmed experimentally.
  • Tested applicationsSuitable for: IHC-Fr, WB, ICC/IF, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Cat, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Rat Cytochrome P450 4A aa 431-445. The immunogen is homologous to rat cytochrome P450 4A2, A10, A12 and A14.
    Sequence:

    DPSRFAPDSPRHSHS

Properties

Applications

Our Abpromise guarantee covers the use of ab3573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
WB 1/200 - 1/2000. Detects a band of approximately 50 kDa.

A slightly larger band representing 4A3 is also clearly visible.

ICC/IF 1/20 - 1/200.
IHC-P 1/100 - 1/500.
IP Use at an assay dependent concentration.

Target

  • FunctionCatalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate, myristate and palmitate. Has little activity toward prostaglandins A1 and E1. Oxidizes arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE).
  • Tissue specificityKidney and liver.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 20-HETE synthase antibody
    • 20-hydroxyeicosatetraenoic acid synthase antibody
    • CP4AB_HUMAN antibody
    • CYP4A antibody
    • Cyp4a1 antibody
    • Cyp4a10 antibody
    • CYP4A11 antibody
    • Cyp4a14 antibody
    • Cyp4a3 antibody
    • CYP4A7 antibody
    • CYP4AII antibody
    • CYPIVA11 antibody
    • Cytochrome P-450HK-omega antibody
    • Cytochrome P450 4A1 antibody
    • Cytochrome P450 4A10 antibody
    • Cytochrome P450 4A11 antibody
    • Cytochrome P450 4A14 antibody
    • Cytochrome P450 4A2 antibody
    • Cytochrome P450 4A3 antibody
    • Cytochrome P450 4A7 antibody
    • Cytochrome P450HL-omega antibody
    • Fatty acid omega-hydroxylase antibody
    • Lauric acid omega-hydroxylase antibody
    see all

Anti-Cytochrome P450 4A antibody images

  • ab3573 labelling Cytochrome P450 4A (green) in the cytoplasm and membrane of H-4-II-E cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3573 labelling Cytochrome P450 4A (green) in the cytoplasm and membrane of HeLa cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3573 labelling Cytochrome P450 4A (green) in the cytoplasm and membrane of PC12 cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3573 labelling Cytochrome P450 4A in the cytoplasm of Rat liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3573 labelling Cytochrome P450 4A in the cytoplasm of Rat kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3573 labelling Cytochrome P450 4A in the cytoplasm and membrane of Human liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

     

References for Anti-Cytochrome P450 4A antibody (ab3573)

This product has been referenced in:
  • Zhang Y  et al. Combined therapy with COX-2 inhibitor and 20-HETE inhibitor reduces colon tumor growth and the adverse effects of ischemic stroke associated with COX-2 inhibition. Am J Physiol Regul Integr Comp Physiol 307:R693-703 (2014). WB ; Mouse . Read more (PubMed: 24990856) »
  • Rector RS  et al. Daily exercise vs. caloric restriction for prevention of nonalcoholic fatty liver disease in the OLETF rat model. Am J Physiol Gastrointest Liver Physiol 300:G874-83 (2011). WB ; Rat . Read more (PubMed: 21350190) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us. For ab23677, LTA4 hydrolase, I suggest either a neutrophil or monocyte lysate. We carry THP1 lysate, ab7913, which is a human monocytic leukemia. For a negative control, LTA4 was detected in epidermal keratinocytes and ...

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I think you must be interested in ab3573 -- Rabbit polyclonal to Cytochrome P450 4A. If so, all the information you need is on the online datasheet under the "immunogen section". Synthetic peptide D(431 PSRFAPDSPRHSHS(445). The immunizing peptide corre...

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Thank you for your enquiry. Ab3573 detects an ~50 kDa protein representing P450 4A in rat liver extract and also detects, to a lesser extent, isoforms 4A1 and 4A3 (in the same rat liver extract sample, a slightly larger band representing 4A3 is also cl...

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