Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
Key features and details
- Rabbit polyclonal to Cytochrome P450 4A/CYP4A11
- Suitable for: IHC-Fr, ICC/IF, IHC-P, WB, IP
- Reacts with: Mouse, Rat, Rabbit, Hamster, Cat, Dog, Human, Pig
- Isotype: IgG
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Overview
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Product name
Anti-Cytochrome P450 4A/CYP4A11 antibody
See all Cytochrome P450 4A/CYP4A11 primary antibodies -
Description
Rabbit polyclonal to Cytochrome P450 4A/CYP4A11 -
Host species
Rabbit -
Specificity
The immunogen is homologous to rat cytochrome P450 4A2, 4A10, 4A12 and 4A14. Gene synonyms are Cyp4a11, Cyp4a1, Cyp4a8, and Cyp4a3, respectively. The specificity to these specific forms has not been confirmed experimentally. Please note nomenclature for specific forms may differ from mouse to rat. -
Tested applications
Suitable for: IHC-Fr, ICC/IF, IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Hamster, Cat, Dog, Human, Pig -
Immunogen
Synthetic peptide corresponding to Rat Cytochrome P450 4A/CYP4A11 aa 400-500. The immunogen is homologous to rat cytochrome P450 4A2, A10, A12 and A14.
Database link: P08516 -
Positive control
- WB: Rat liver tissue lysate, H-4-II-E and HeLa cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Whole antiserum -
Primary antibody notes
The Cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA’s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, & 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic & brain microvessels. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
1/20 - 1/200.
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IHC-P |
1/100 - 1/500.
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WB |
1/200 - 1/2000. Predicted molecular weight: 59 kDa.
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IP |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
1/20 - 1/200. |
IHC-P
1/100 - 1/500. |
WB
1/200 - 1/2000. Predicted molecular weight: 59 kDa. |
IP
Use at an assay dependent concentration. |
Target
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Function
Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate, myristate and palmitate. Has little activity toward prostaglandins A1 and E1. Oxidizes arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE). -
Tissue specificity
Kidney and liver. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1579 Human
- Entrez Gene: 24306 Rat
- Entrez Gene: 298423 Rat
- Entrez Gene: 50549 Rat
- Omim: 601310 Human
- SwissProt: Q02928 Human
- SwissProt: P14581 Rabbit
- SwissProt: P08516 Rat
see all -
Alternative names
- 20-HETE synthase antibody
- 20-hydroxyeicosatetraenoic acid synthase antibody
- CP4AB_HUMAN antibody
see all
Images
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All lanes : Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573) at 1/1000 dilution
Lane 1 : Rat liver tissue lysate
Lane 2 : H-4-II-E cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 59 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?Chemiluminescent detection
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ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of H-4-II-E (rat) cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm of Rat liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of HeLa cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of PC12 cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm of Rat kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm and membrane of Human liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab3573 has been referenced in 15 publications.
- Cui W et al. 20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1a pathway: A translational study. Cell Prolif 54:e12964 (2021). PubMed: 33314534
- Deguise MO et al. SMN Depleted Mice Offer a Robust and Rapid Onset Model of Nonalcoholic Fatty Liver Disease. Cell Mol Gastroenterol Hepatol 12:354-377.e3 (2021). PubMed: 33545428
- Han X et al. 20-HETE synthesis inhibition promotes cerebral protection after intracerebral hemorrhage without inhibiting angiogenesis. J Cereb Blood Flow Metab 39:1531-1543 (2019). PubMed: 29485354
- Lai YJ et al. Estrogen receptor a promotes Cav1.2 ubiquitination and degradation in neuronal cells and in APP/PS1 mice. Aging Cell N/A:e12961 (2019). PubMed: 31012223
- Li H et al. Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction through AA Metabolism Modulated CYPs Pathway. Int J Biol Sci 15:2037-2050 (2019). PubMed: 31592228