Anti-Cytochrome P450 Reductase antibody (ab13513)
Key features and details
- Rabbit polyclonal to Cytochrome P450 Reductase
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-Cytochrome P450 Reductase antibody
See all Cytochrome P450 Reductase primary antibodies -
Description
Rabbit polyclonal to Cytochrome P450 Reductase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Full length native protein (purified) corresponding to Rat Cytochrome P450 Reductase.
Database link: P00388 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/1000. Detects a band of approximately 78 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/1000. Detects a band of approximately 78 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. -
Involvement in disease
Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750]. -
Sequence similarities
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
Cellular localization
Endoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region. - Information by UniProt
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Database links
- Entrez Gene: 5447 Human
- Entrez Gene: 29441 Rat
- Omim: 124015 Human
- SwissProt: P16435 Human
- SwissProt: P00388 Rat
- Unigene: 354056 Human
- Unigene: 11359 Rat
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Alternative names
- CPR antibody
- CYPOR antibody
- Cytochrome p450 oxidoreductase antibody
see all
Images
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Anti-Cytochrome P450 Reductase antibody (ab13513) at 1/2000 dilution + Cell lysates prepared from rat PC12 cells
Western blot analysis of rat PC12 cell lysate, probed with ab13513. -
ICC/IF image of ab13513 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13513, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab13513 has been referenced in 23 publications.
- Kumondai M et al. Heterologous expression of high-activity cytochrome P450 in mammalian cells. Sci Rep 10:14193 (2020). PubMed: 32843676
- Chidawanyika T et al. A Genome-Wide CRISPR/Cas9 Screen Reveals that Riboflavin Regulates Hydrogen Peroxide Entry into HAP1 Cells. mBio 11:N/A (2020). PubMed: 32788383
- Froeling FEM et al. Bioactivation of Napabucasin Triggers Reactive Oxygen Species-Mediated Cancer Cell Death. Clin Cancer Res 25:7162-7174 (2019). PubMed: 31527169
- Yao Y et al. Effects and mechanism of amyloid ß1-42 on mitochondria in astrocytes. Mol Med Rep 17:6997-7004 (2018). PubMed: 29568933
- Pankowicz FP et al. Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing. Gastroenterology 155:1967-1970.e6 (2018). PubMed: 30170115