Anti-Cytochrome P450 Reductase antibody (ab89622)


  • Product nameAnti-Cytochrome P450 Reductase antibody
    See all Cytochrome P450 Reductase primary antibodies
  • Description
    Mouse polyclonal to Cytochrome P450 Reductase
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human protein (AAH34277.1)

  • Positive control
    • WB: liver lysate. ICC/IF: HeLa cells.



Our Abpromise guarantee covers the use of ab89622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa.
ICC/IF Use a concentration of 10 µg/ml.


  • FunctionThis enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
  • Involvement in diseaseDefects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
    Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750].
  • Sequence similaritiesIn the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Cellular localizationEndoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region.
  • Information by UniProt
  • Database links
  • Alternative names
    • CPR antibody
    • CYPOR antibody
    • DKFZp686G04235 antibody
    • FLJ26468 antibody
    • NADPH Cytochrome P450 Reductase antibody
    • NADPH dependent cytochrome P450 reductase antibody
    • NADPH--cytochrome P450 reductase antibody
    • NCPR_HUMAN antibody
    • P450 (cytochrome) oxidoreductase antibody
    • P450 Cytochrome Oxidoreductase antibody
    • P450R antibody
    • por antibody
    see all

Anti-Cytochrome P450 Reductase antibody images

  • Anti-Cytochrome P450 Reductase antibody (ab89622) at 1 µg/ml + Human liver lysate at 50 µg

    Predicted band size : 77 kDa
  • All lanes : Anti-Cytochrome P450 Reductase antibody (ab89622) at 1 µg/ml

    Lane 1 : Cytochrome P450 Reductase transfected 293T cell lysate
    Lane 2 : Untransfected lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 77 kDa
  • Immunofluorescence analysis of Cytochrome P450 Reductase expression in PFA-fixed HeLa cells using ab89622 at 10 µg/ml.

References for Anti-Cytochrome P450 Reductase antibody (ab89622)

ab89622 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (HEK283T)
Loading amount 200000 cells
Specification HEK283T
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Jun 20 2012

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I am very pleased to hear you would like to accept our offer and test ab89622 in mouse tissue. You are correct that you have to purchase the antibody ab89622 first, and then after submit...

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Thank you very much for your interest in ab89622.
We do not provide sample sizes of our antibodies but as we have not tested ab89622 in mouse tissue (although there is a 92% level of homology between human an mouse, so there is a very good chance ...

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