Recombinant
RabMAb

Anti-Cytohesin 1 antibody [EPR10050] (ab154847)

Overview

  • Product name
    Anti-Cytohesin 1 antibody [EPR10050]
    See all Cytohesin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10050] to Cytohesin 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cytohesin 1 (Uniprot: Q15438)

  • Positive control
    • Raji, Jurkat and HuT-78 cell lysates; Human colon tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10050
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Contains 1 PH domain.
      Contains 1 SEC7 domain.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • B2 1 antibody
      • CLM1 antibody
      • CTH 1 antibody
      • CYH1_HUMAN antibody
      • CYTH1 antibody
      • CYTIP antibody
      • Cytoadhesin 1 antibody
      • CYTOHESIN 1 antibody
      • Cytohesin-1 antibody
      • D17S811E antibody
      • FLJ34050 antibody
      • FLJ41900 antibody
      • Homolog of secretory protein SEC7 antibody
      • KIAA4240 antibody
      • mKIAA4240 antibody
      • mSec7 1 antibody
      • OTTHUMP00000196778 antibody
      • OTTHUMP00000196779 antibody
      • PH antibody
      • PH, SEC7 and coiled-coil domain containing protein 1 antibody
      • Pleckstrin homology Sec7 and coiled/coil domains 1 antibody
      • Pleckstrin homology Sec7 and coiled/coil domains protein 1 antibody
      • Pleckstrin homology, Sec7 and coiled coil domains 1 antibody
      • PSCD1 antibody
      • SEC7 and coiled-coil domain-containing protein 1 antibody
      • Sec7 antibody
      • SEC7 homolog A antibody
      • SEC7 homolog B2 1 antibody
      • SEC7 homolog B2-1 antibody
      • SEC7, yeast, homolog of antibody
      • Sec7a antibody
      see all

    Images

    • All lanes : Anti-Cytohesin 1 antibody [EPR10050] (ab154847) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HuT-78 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 46 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling Cytohesin 1 using ab154847 at a 1/50 dilution.

    References

    ab154847 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab154847.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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