Cytohesin 1 overexpression 293T lysate (whole cell) (ab94252)

Overview

  • Product nameCytohesin 1 overexpression 293T lysate (whole cell)
  • Description
    Cytohesin 1 overexpression lysate
  • General notesab94252 is a 293T cell transfected lysate in which Human Cytohesin 1 has been transiently over-expressed using a pCMV-Cytohesin 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundFunction: Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP. Tissue specificity: Ubiquitous. Similarity: Contains 1 PH domain. Contains 1 SEC7 domain.

Applications

Our Abpromise guarantee covers the use of ab94252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Cytohesin 1 overexpression 293T lysate (whole cell) images

  • ab94252 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-Cytohesin 1 antibody (ab56511) at 1/500 dilution

    Lane 1 : Cytohesin 1 overexpression 293T lysate (whole cell) (ab94252)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Cytohesin 1 overexpression 293T lysate (whole cell) (ab94252)

ab94252 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"