General notesab94253 is a 293T cell transfected lysate in which Human Cytohesin 2 has been transiently over-expressed using a pCMV-Cytohesin 2 plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more details about how the transfected lysate was prepared view preparation notes
BackgroundFunction: Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane.
Tissue specificity: Ubiquitous.
Similarity: Contains 1 PH domain.
Contains 1 SEC7 domain.
Domain: The PH domain is necessary and sufficient for plasma membrane relocalization.