Cytohesin 2 overexpression 293T lysate (whole cell) (ab94253)

Overview

  • Product nameCytohesin 2 overexpression 293T lysate (whole cell)
  • Description
    Cytohesin 2 overexpression lysate
  • General notesab94253 is a 293T cell transfected lysate in which Human Cytohesin 2 has been transiently over-expressed using a pCMV-Cytohesin 2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Tissue specificity: Ubiquitous. Similarity: Contains 1 PH domain. Contains 1 SEC7 domain. Domain: The PH domain is necessary and sufficient for plasma membrane relocalization.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Cytohesin 2 overexpression 293T lysate (whole cell) images

  • ab94253 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-Cytohesin 2 antibody (ab56510) at 1/500 dilution

    Lane 1 : Cytohesin 2 overexpression 293T lysate (whole cell) (ab94253)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Cytohesin 2 overexpression 293T lysate (whole cell) (ab94253)

ab94253 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"