Anti-Cytokeratin 1 - 8 antibody [AE3], prediluted (ab34255)

Overview

  • Product nameAnti-Cytokeratin 1 - 8 antibody [AE3], prediluted
    See all Cytokeratin 1 - 8 primary antibodies
  • Description
    Mouse monoclonal [AE3] to Cytokeratin 1 - 8, prediluted
  • Specificityab32455 reacts with most of the Basic (Type II) keratins. Including epidermal cell keratin of 86 and 58kD and skin at 58kD, 65kD and 67kD. Broad keratin reactivity is found on human epidermal cell, oesophagus and cornea. Since each epithelium contains at least one basic keratin, AE3 is broadly reactive and stains positively almost all epithelia and their neoplasms.
  • Tested applicationsSuitable for: IHC-FoFr, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Fish, Monkey, Macaque Monkey
  • Immunogen

    Tissue/ cell preparation - Human epidermal cells.

  • Positive control
    • Human epidermal cells, oesophagus, cornea.

Properties

Applications

Our Abpromise guarantee covers the use of ab34255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
IHC-P
IHC-Fr
  • Application notesIHC-Fr: Use neat
    IHC-FoFr: Use neat.
    IHC-P: Use neat. This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References for Anti-Cytokeratin 1 - 8 antibody [AE3], prediluted (ab34255)

    This product has been referenced in:
    • Tsubura A  et al. Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species. Acta Anat (Basel) 140:287-93 (1991). Read more (PubMed: 1714223) »
    • Eichner R  et al. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J Cell Biol 98:1388-96 (1984). Read more (PubMed: 6201491) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Chicken Tissue sections (chicken embryo epicardium)
    Specification chicken embryo epicardium
    Fixative Paraformaldehyde
    Permeabilization Yes - 0,2% Triton X-100 in PBS, 10'
    Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Mrs. Elena Astanina

    Verified customer

    Submitted Feb 20 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"