Anti-Cytokeratin 1 antibody [AE1] (ab9286)
Key features and details
- Mouse monoclonal [AE1] to Cytokeratin 1
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Monkey, Turtle
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cytokeratin 1 antibody [AE1]
See all Cytokeratin 1 primary antibodies -
Description
Mouse monoclonal [AE1] to Cytokeratin 1 -
Host species
Mouse -
Specificity
This antibody recognises most of the acidic (Type 1) keratins. -
Tested applications
Suitable for: IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Monkey, Turtle -
Immunogen
Full length native protein (purified) corresponding to Human Cytokeratin 1. Epidermal keratin
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
AE1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 66 kDa. |
Target
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Function
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). -
Tissue specificity
The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis. -
Involvement in disease
Defects in KRT1 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
Defects in KRT1 are the cause of ichthyosis hystrix Curth-Macklin type (IHCM) [MIM:146590]. IHCM is a genodermatosis with severe verrucous hyperkeratosis. Affected individuals manifest congenital verrucous black scale on the scalp, neck, and limbs with truncal erythema, palmoplantar keratoderma and keratoses on the lips, ears, nipples and buttocks.
Defects in KRT1 are a cause of palmoplantar keratoderma non-epidermolytic (NEPPK) [MIM:600962]. NEPKK is a dermatological disorder characterized by focal palmoplantar keratoderma with oral, genital, and follicular lesions.
Defects in KRT1 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
Defects in KRT1 are the cause of palmoplantar keratoderma striate type 3 (SPPK3) [MIM:607654]; also known as keratosis palmoplantaris striata III. SPPK3 is a dermatological disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsUndergoes deimination of some arginine residues (citrullination). -
Cellular localization
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells. - Information by UniProt
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Database links
- Entrez Gene: 100301161 Cow
- Entrez Gene: 281266 Cow
- Entrez Gene: 16678 Mouse
- Entrez Gene: 300250 Rat
- SwissProt: P04104 Mouse
- SwissProt: Q6IMF3 Rat
- Unigene: 183137 Mouse
- Unigene: 31789 Rat
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Alternative names
- 67 kDa cytokeratin antibody
- CK-1 antibody
- CK1 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab9286 has been referenced in 13 publications.
- Choo MK et al. The protein kinase p38a destabilizes p63 to limit epidermal stem cell frequency and tumorigenic potential. Sci Signal 11:N/A (2018). PubMed: 30301786
- Fang H et al. Proinflammatory role of blister fluid-derived exosomes in bullous pemphigoid. J Pathol 245:114-125 (2018). PubMed: 29468680
- Yin C et al. TLR7-expressing cells comprise an interfollicular epidermal stem cell population in murine epidermis. Sci Rep 4:5831 (2014). PubMed: 25060222
- Caballero-Franco C et al. Tuning of protein kinase circuitry by p38a is vital for epithelial tissue homeostasis. J Biol Chem 288:23788-97 (2013). PubMed: 23836897
- Spagnolo DV et al. Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms. Am J Clin Pathol 84:697-704 (1985). PubMed: 2416215