The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/1000.
1/300 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Seen in all suprabasal cell layers including stratum corneum.
Involvement in disease
Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop. Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
All lanes : Anti-Cytokeratin 10 antibody (ab53124) at 1/300 dilution
Lane 1 : HeLa cell extract Lane 2 : HeLa cell extract with immunising peptide
Predicted band size : 60 kDa Observed band size : 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody (ab53124)Image from Kawabata K et al., Biochim Biophys Acta, 1246-52, 25 June 2011. Fig 4.; doi:10.1016/j.bbapap.2011.06.006
Immunohistochemical analysis of Human abdominal skin tissue, staining Cytokeratin 10 with ab53124.
Sections were blocked with 10% normal goat serum in BSA/PBS-T for 20 minutes at room temperature. Samples were incubated with primary antibody (1/1000) in BSA/PBS-T overnight at 4°C. A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.
Butovich IA et al. Biophysical and morphological evaluation of human normal and dry eye meibum using hot stage polarized light microscopy. Invest Ophthalmol Vis Sci55:87-101 (2014).
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Kawabata K et al. The presence of N(e)-(Carboxymethyl) lysine in the human epidermis. Biochim Biophys Acta1814:1246-52 (2011).
Read more (PubMed: 21708295) »