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Recombinant protein fragment corresponding to a region within amino acids 23 and 284 of Human Cytokeratin 10 (NP_000412).
Our Abpromise guarantee covers the use of ab97764 in the following tested applications.
|WB||1/500 - 1/3000. Predicted molecular weight: 59 kDa.|
|IP||Use a concentration of 5 µg/ml.|
Cytokeratin 10 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Cytokeratin 10 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab97764.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 59kDa; Cytokeratin 10, non specific bands - 52kDa and in control (lane 2): We are unsure as to the identity of this extra band.