Anti-Cytokeratin 10 antibody [DE-K10] (ab9026)

Overview

  • Product nameAnti-Cytokeratin 10 antibody [DE-K10]
    See all Cytokeratin 10 primary antibodies
  • Description
    Mouse monoclonal [DE-K10] to Cytokeratin 10
  • SpecificityReacts with keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.
  • Tested applicationsSuitable for: IHC-Fr, ICC, IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Sheep, Cat, Dog, Human
  • Immunogen

    Cytoskeletal preparation extracted from human epidermis

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberDE-K10
  • MyelomaSp2/0
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/100.
WB 1/100 - 1/1000.
Flow Cyt 1/100 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Tissue specificitySeen in all suprabasal cell layers including stratum corneum.
  • Involvement in diseaseDefects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCIE antibody
    • BIE antibody
    • CK 10 antibody
    • CK-10 antibody
    • Cytokeratin-10 antibody
    • EHK antibody
    • K10 antibody
    • K1C10_HUMAN antibody
    • Keratin 10 antibody
    • Keratin antibody
    • Keratin type i cytoskeletal 10 antibody
    • Keratin type I cytoskeletal 59 kDa antibody
    • Keratin-10 antibody
    • Keratin10 antibody
    • KPP antibody
    • KRT10 antibody
    • type I cytoskeletal 10 antibody
    see all

Anti-Cytokeratin 10 antibody [DE-K10] images

  • IHC image of ab9026 staining in Human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9026, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Mesenchymal-epidermal interactions assessed by heterologous organotypic co-cultures (OTC). Heterologous OTC consisting of mice deficient for cathepsin L +/+ fibroblasts in collagen type I gels topped by normal human primary keratinocytes were grown air-exposed for 7 days. Paraffin sections were stained in haematoxylin and eosin (HE) or by immunohistochemistry for the proliferation marker Ki67 (Ki67, brown nuclear staining) and the differentiation markers cytokeratin 10 (K10, ab9026 1/100 dilution, brown staining) and transglutaminase (TG, brown staining). Bar 100 µm.

References for Anti-Cytokeratin 10 antibody [DE-K10] (ab9026)

This product has been referenced in:
  • Pearson HB  et al. The polarity protein Scrib mediates epidermal development and exerts a tumor suppressive function during skin carcinogenesis. Mol Cancer 14:169 (2015). Read more (PubMed: 26376988) »
  • Varone A  et al. Endogenous two-photon fluorescence imaging elucidates metabolic changes related to enhanced glycolysis and glutamine consumption in precancerous epithelial tissues. Cancer Res 74:3067-75 (2014). Read more (PubMed: 24686167) »

See all 12 Publications for this product

Product Wall

Application Western blot
Sample Pig Tissue lysate - whole (skin)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Treatment nurn wound with stem cell treatment
Specification skin
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted May 20 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.05% typsin
Sample Human Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde
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Verified customer

Submitted Feb 23 2015

I have just received the following information from the supplier of ab9026: "1) Yes please, you can offer your customer a free (replacement) batch of our other keratin 10 ab9025. 2)The question is however, whether this will be of any help. Ab902...

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I'm sorry to hear you are having problem with ab9026. Spotting can be frequently caused by the blocking agent you use, I would therefore try filtering it and leaving on the membrane for 30min, then rinsing the membrane with TBST. Incubate the prima...

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The exact epitope of Ab9026 is not known. I'm afraid you just have to try and see if your truncated protein is still recognized by Ab9026.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"