Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)
Key features and details
- Mouse monoclonal [DE-K10] to Cytokeratin 10 - Cytoskeleton Marker
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Dog, Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker
See all Cytokeratin 10 primary antibodies -
Description
Mouse monoclonal [DE-K10] to Cytokeratin 10 - Cytoskeleton Marker -
Host species
Mouse -
Specificity
Reacts with keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas. -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Dog, Human -
Immunogen
Tissue, cells or virus corresponding to Human Cytokeratin 10. Cytoskeletal preparation extracted from human epidermis
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
DE-K10 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P | (3) |
1/100.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
1/100. |
Target
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Tissue specificity
Seen in all suprabasal cell layers including stratum corneum. -
Involvement in disease
Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 491006 Dog
- Entrez Gene: 3858 Human
- Omim: 148080 Human
- SwissProt: Q6EIZ0 Dog
- SwissProt: P13645 Human
- Unigene: 99936 Human
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Alternative names
- BCIE antibody
- BIE antibody
- CK 10 antibody
see all
Images
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IHC image on a frozen section of dog skin showing its strong reactivity in the keratinizing epidermal cells.
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IHC image of ab9026 staining in Human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9026, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Mesenchymal-epidermal interactions assessed by heterologous organotypic co-cultures (OTC). Heterologous OTC consisting of mice deficient for cathepsin L +/+ fibroblasts in collagen type I gels topped by normal human primary keratinocytes were grown air-exposed for 7 days. Paraffin sections were stained in haematoxylin and eosin (HE) or by immunohistochemistry for the proliferation marker Ki67 (Ki67, brown nuclear staining) and the differentiation markers cytokeratin 10 (K10, ab9026 1/100 dilution, brown staining) and transglutaminase (TG, brown staining). Bar 100 µm.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab9026 has been referenced in 40 publications.
- Koren E et al. Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis. Nat Commun 13:4628 (2022). PubMed: 35941116
- Ghuwalewala S et al. Binary organization of epidermal basal domains highlights robustness to environmental exposure. EMBO J 41:e110488 (2022). PubMed: 35949182
- Mini CA et al. Immortalized keratinocytes cells generates an effective model of Epidermal Human Equivalent for irritation and corrosion tests. Toxicol In Vitro 71:105069 (2021). PubMed: 33309870
- Kitazawa K et al. Chondroitin 6-sulfate represses keratinocyte proliferation in mouse skin, which is associated with psoriasis. Commun Biol 4:114 (2021). PubMed: 33495490
- Abreu CM et al. Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages. Bioeng Transl Med 6:e10195 (2021). PubMed: 34027085