Anti-Cytokeratin 13 antibody (ab79279)
Key features and details
- Goat polyclonal to Cytokeratin 13
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 13 antibody
See all Cytokeratin 13 primary antibodies -
Description
Goat polyclonal to Cytokeratin 13 -
Host species
Goat -
Specificity
ab79279 is expected to recognize the reported isoform a (NP_705694.2) only. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
C-TSNASGRRTSDVRRP
, corresponding to C terminal amino acids 444-458 of Human Cytokeratin 13 (NP_705694.2) -
Positive control
- Human Lung, Skeletal Muscle and Skin lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab79279 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Tissue specificity
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. -
Involvement in disease
Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsO-glycosylated; glycans consist of single N-acetylglucosamine residues. - Information by UniProt
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Database links
- Entrez Gene: 3860 Human
- Omim: 148065 Human
- SwissProt: P13646 Human
- Unigene: 654550 Human
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Alternative names
- 47 kDa cytokeratin antibody
- CK-13 antibody
- CK13 antibody
see all
Images
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Anti-Cytokeratin 13 antibody (ab79279) at 0.1 µg/ml + Human Lung lysate (in RIPA buffer) at 35 µg
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan, Microbiology. 2003 Apr;149(Pt 4):961-71.; PMID: 12686638)
Datasheets and documents
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Datasheet download
References (2)
ab79279 has been referenced in 2 publications.
- Tadokoro T et al. Dorso-ventral heterogeneity in tracheal basal stem cells. Biol Open 10:N/A (2021). PubMed: 34396394
- Montoro DT et al. A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature 560:319-324 (2018). PubMed: 30069044