Anti-Cytokeratin 13 antibody [AE8] (ab16112)

Overview

  • Product nameAnti-Cytokeratin 13 antibody [AE8]
    See all Cytokeratin 13 primary antibodies
  • Description
    Mouse monoclonal [AE8] to Cytokeratin 13
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Human
  • Immunogen

    Rabbit esophageal keratins.

  • General notes


    This antibody is specific for Cytokeratin 13, which is a marker for oesophageal type differentiation which is expressed by various internal stratified epithelia.

Properties

Applications

Our Abpromise guarantee covers the use of ab16112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 25076852
WB 1/1000 - 1/3000. Predicted molecular weight: 50 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Tissue specificityExpressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
  • Involvement in diseaseDefects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    O-glycosylated; glycans consist of single N-acetylglucosamine residues.
  • Information by UniProt
  • Database links
  • Alternative names
    • 47 kDa cytokeratin antibody
    • CK-13 antibody
    • CK13 antibody
    • Cytokeratin 13 antibody
    • Cytokeratin-13 antibody
    • K13 antibody
    • K1C13_HUMAN antibody
    • Ka13 antibody
    • Keratin 13 antibody
    • Keratin antibody
    • keratin type I cytoskeletal 13 antibody
    • Keratin-13 antibody
    • Krt-1.13 antibody
    • Krt1-13 antibody
    • KRT13 antibody
    • MGC161462 antibody
    • MGC3781 antibody
    • type I cytoskeletal 13 antibody
    • Type I keratin Ka13 antibody
    • WSN2 antibody
    see all

Anti-Cytokeratin 13 antibody [AE8] images

  • ab16112 staining Cytokeratin 13 in Human pharynx tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in Tris pH9. Samples were incubated with primary antibody (undiluted) for 1 hour at 20°C. An undiluted HRP-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Cytokeratin 13 antibody [AE8] (ab16112)

This product has been referenced in:
  • Okuda Y  et al. Wnt signaling as a possible promoting factor of cell differentiation in pleomorphic adenomas. Int J Med Sci 11:971-8 (2014). ICC/IF ; Human . Read more (PubMed: 25076852) »
  • Rival-Gervier S  et al. Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element. Mol Ther 21:1536-50 (2013). Read more (PubMed: 23752310) »

See all 10 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH9
Sample Human Tissue sections (pharynx)
Specification pharynx
Permeabilization No
Fixative Formaldehyde
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Verified customer

Submitted Oct 29 2013

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