Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)


  • Product name
    Anti-Cytokeratin 13 antibody [EPR3671]
    See all Cytokeratin 13 primary antibodies
  • Description
    Rabbit monoclonal [EPR3671] to Cytokeratin 13
  • Host species
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Cytokeratin 13 aa 100-200. The exact sequence is proprietary.
    (Peptide available as ab180923)

  • Positive control
    • WB: A431, HACAT or 293T cell lysate. IHC: transitional cell urinary bladder carcinoma tissue. FC: A431 cells
  • General notes


    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab92551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/100000 - 1/500000. Predicted molecular weight: 50 kDa.Can be blocked with Cytokeratin 13 peptide (ab180923).
ICC 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/20.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Tissue specificity
      Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
    • Involvement in disease
      Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Post-translational
      O-glycosylated; glycans consist of single N-acetylglucosamine residues.
    • Information by UniProt
    • Database links
    • Alternative names
      • 47 kDa cytokeratin antibody
      • CK-13 antibody
      • CK13 antibody
      • Cytokeratin 13 antibody
      • Cytokeratin-13 antibody
      • K13 antibody
      • K1C13_HUMAN antibody
      • Ka13 antibody
      • Keratin 13 antibody
      • Keratin antibody
      • keratin type I cytoskeletal 13 antibody
      • Keratin-13 antibody
      • Krt-1.13 antibody
      • Krt1-13 antibody
      • KRT13 antibody
      • MGC161462 antibody
      • MGC3781 antibody
      • type I cytoskeletal 13 antibody
      • Type I keratin Ka13 antibody
      • WSN2 antibody
      see all


    • All lanes : Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) at 1/100000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HACAT cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Lanes 1-2 : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
      Lane 3 : HRP labelled goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 50 kDa

    • ab92551 at 1/100 dilution staining Cytokeratin 13 in formalin-fixed, paraffin-embedded transitional cell usrinary bladder carcinoma tissue by immunohistochemistry. Detection: DAB staining.
    • ab92551 staining Cytokeratin 13 in Human vaginal epithelium tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton in PBS and blocked with 1% fish skin gelatin for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
    • Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 13 with purified ab92551 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • ab92551 showing positive staining in Cervical carcinoma tissue.

    • ab92551 showing positive staining in Normal tonsil squamous cells tissue.

    • ab92551 showing negative staining in Glioma tissue.

    • ab92551 showing negative staining in Breast carcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Liu S  et al. Keratin 13 Is Enriched in Prostate Tubule-Initiating Cells and May Identify Primary Prostate Tumors that Metastasize to the Bone. PLoS One 11:e0163232 (2016). IHC ; Human . Read more (PubMed: 27711225) »
    • Palazzo E  et al. The homeoprotein DLX3 and tumor suppressor p53 co-regulate cell cycle progression and squamous tumor growth. Oncogene 35:3114-24 (2016). IHC . Read more (PubMed: 26522723) »

    See all 10 Publications for this product

    Customer reviews and Q&As

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    This product because it is IVD has not been imported to Abcam website. Epitomics still sell this product via their website. Please contact them at the following email address;
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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunohistochemistry (Frozen sections)
    Human Tissue sections (vaginal epithelium)
    vaginal epithelium
    Yes - 0.2% Triton in PBS
    Blocking step
    (agent) for 30 minute(s) · Concentration: 1% · Temperature: 25°C

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    Verified customer

    Submitted Oct 19 2011


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