Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

Overview

  • Product nameAnti-Cytokeratin 13 antibody [EPR3671]
    See all Cytokeratin 13 primary antibodies
  • Description
    Rabbit monoclonal [EPR3671] to Cytokeratin 13
  • Tested applicationsSuitable for: IHC-Fr, WB, ICC, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • WB: A431, HACAT or 293T cell lysate. IHC: transitional cell urinary bladder carcinoma tissue.
  • General notes

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/100000 - 1/500000. Predicted molecular weight: 50 kDa.Can be blocked with Cytokeratin 13 peptide (ab180923).
ICC 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • Tissue specificityExpressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
    • Involvement in diseaseDefects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Post-translational
      modifications
      O-glycosylated; glycans consist of single N-acetylglucosamine residues.
    • Information by UniProt
    • Database links
    • Alternative names
      • 47 kDa cytokeratin antibody
      • CK-13 antibody
      • CK13 antibody
      • Cytokeratin 13 antibody
      • Cytokeratin-13 antibody
      • K13 antibody
      • K1C13_HUMAN antibody
      • Ka13 antibody
      • Keratin 13 antibody
      • Keratin antibody
      • keratin type I cytoskeletal 13 antibody
      • Keratin-13 antibody
      • Krt-1.13 antibody
      • Krt1-13 antibody
      • KRT13 antibody
      • MGC161462 antibody
      • MGC3781 antibody
      • type I cytoskeletal 13 antibody
      • Type I keratin Ka13 antibody
      • WSN2 antibody
      see all

    Anti-Cytokeratin 13 antibody [EPR3671] images

    • All lanes : Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) at 1/100000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HACAT cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size : 50 kDa
    • ab92551 at 1/100 dilution staining Cytokeratin 13 in formalin-fixed, paraffin-embedded transitional cell usrinary bladder carcinoma tissue by immunohistochemistry. Detection: DAB staining.
    • ab92551 staining Cytokeratin 13 in Human vaginal epithelium tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton in PBS and blocked with 1% fish skin gelatin for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
    • ab92551 showing positive staining in Cervical carcinoma tissue.

    • ab92551 showing positive staining in Normal tonsil squamous cells tissue.

    • ab92551 showing negative staining in Glioma tissue.

    • ab92551 showing negative staining in Breast carcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    This product has been referenced in:
    • Montagner A  et al. Src is activated by the nuclear receptor peroxisome proliferator-activated receptor ß/d in ultraviolet radiation-induced skin cancer. EMBO Mol Med 6:80-98 (2014). IF, IHC . Read more (PubMed: 24203162) »
    • Yan M  et al. IKKa restoration via EZH2 suppression induces nasopharyngeal carcinoma differentiation. Nat Commun 5:3661 (2014). Read more (PubMed: 24739462) »

    See all 5 Publications for this product

    Product Wall

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    This product because it is IVD has not been imported to Abcam website. Epitomics still sell this product via their website. Please contact them at the following email address; mailto:IVDorders@epitomics.com
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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (vaginal epithelium)
    Specification vaginal epithelium
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2% Triton in PBS
    Blocking step (agent) for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Oct 19 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"