Anti-Cytokeratin 15 antibody [EPR1614Y] (HRP) (ab194067)


  • Product name
    Anti-Cytokeratin 15 antibody [EPR1614Y] (HRP)
    See all Cytokeratin 15 primary antibodies
  • Description
    Rabbit monoclonal [EPR1614Y] to Cytokeratin 15 (HRP)
  • Host species
  • Conjugation
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues from the C-terminus of human Cytokeratin 15.

  • Positive control
    • WB: Human Fetal thymus tissue lysate. IHC: normal human skin tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab194067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.


  • Tissue specificity
    Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI528832 antibody
    • CK 15 antibody
    • CK-15 antibody
    • CK15 antibody
    • Cytokeratin-15 antibody
    • Cytokeratin15 antibody
    • K15 antibody
    • K1C15_HUMAN antibody
    • K1CO antibody
    • Ka15 antibody
    • Keratin 15 antibody
    • Keratin 15 basic antibody
    • Keratin 15 beta antibody
    • Keratin antibody
    • Keratin complex 1 acidic gene 15 antibody
    • Keratin type I cytoskeletal 15 antibody
    • Keratin-15 antibody
    • Keratin15 antibody
    • KRT 15 antibody
    • Krt1-15 antibody
    • KRT15 antibody
    • KRTB antibody
    • KRTL15 antibody
    • Type I cytoskeletal 15 antibody
    • Type I keratin Ka15 antibody
    see all


  • Anti-Cytokeratin 15 antibody [EPR1614Y] (HRP) (ab194067) at 1/5000 dilution + Thymus (Human) Whole Cell Lysate - fetal normal tissue at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 52 kDa (why is the actual band size different from the predicted?)

    Exposure time: 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab194067 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of Cytokeratin 15 staining in a section of formalin-fixed paraffin-embedded normal human skin tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab194067, at a working dilution of 1 in 500 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre


ab194067 has not yet been referenced specifically in any publications.

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