Overview

  • Product name
    Anti-Cytokeratin 16 antibody
    See all Cytokeratin 16 primary antibodies
  • Description
    Rabbit polyclonal to Cytokeratin 16
  • Specificity
    ab53117 detects endogenous levels of total Cytokeratin 16 protein.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide derived from human Cytokeratin 16.

  • Positive control
    • Human breast carcinoma tissue and HepG2 cell extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/300 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • Tissue specificity
    Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
  • Involvement in disease
    Defects in KRT16 are a cause of pachyonychia congenita type 1 (PC1) [MIM:167200]; also known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present.
    Defects in KRT16 are the cause of palmoplantar keratoderma non-epidermolytic focal (FNEPPK) [MIM:613000]. A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present.
    Defects in KRT16 are a cause of unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]. UPVN is characterized by a localized thickening of the skin in parts of the right palm and the right sole.
    Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • CK 16 antibody
    • CK-16 antibody
    • CK16 antibody
    • Cytokeratin-16 antibody
    • Cytokeratin16 antibody
    • FNEPPK antibody
    • Focal non epidermolytic palmoplantar keratoderma antibody
    • K 16 antibody
    • K16 antibody
    • K1C16_HUMAN antibody
    • K1CP antibody
    • Keratin 1 type I antibody
    • Keratin 16 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 16 antibody
    • Keratin-16 antibody
    • Keratin16 antibody
    • KRT 16 antibody
    • Krt16 antibody
    • KRT16A antibody
    • NEPPK antibody
    • PC1 antibody
    • type I cytoskeletal 16 antibody
    see all

Anti-Cytokeratin 16 antibody images

  • All lanes : Anti-Cytokeratin 16 antibody (ab53117) at 1/300 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunising peptide


    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • ab53117 at 1/50 dilution staining Cytokeratin 16 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.ab53117 at 1/50 dilution staining Cytokeratin 16 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • ICC/IF image of ab53117 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53117, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab53117 staining Cytokeratin 16 in transplanted Human skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 5% Goat serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour at 22°C. An HRP-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-Cytokeratin 16 antibody (ab53117)

This product has been referenced in:
  • Gendronneau G  et al. Overexpression of galectin-7 in mouse epidermis leads to loss of cell junctions and defective skin repair. PLoS One 10:e0119031 (2015). Read more (PubMed: 25741714) »
  • Tortelli F  et al. Fibronectin binding modulates CXCL11 activity and facilitates wound healing. PLoS One 8:e79610 (2013). Read more (PubMed: 24205388) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (transplanted human skin)
Specification
transplanted human skin
Fixative
Acetone
Permeabilization
No
Blocking step
Goat serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Graham Elliott

Verified customer

Submitted Aug 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up