The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Ready to use.
Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityExpressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
Involvement in diseaseDefects in KRT16 are a cause of pachyonychia congenita type 1 (PC1) [MIM:167200]; also known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present. Defects in KRT16 are the cause of palmoplantar keratoderma non-epidermolytic focal (FNEPPK) [MIM:613000]. A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present. Defects in KRT16 are a cause of unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]. UPVN is characterized by a localized thickening of the skin in parts of the right palm and the right sole. Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
Sequence similaritiesBelongs to the intermediate filament family.
ab75125, at 1µg/ml, staining Cytokeratin 16 formalin fixed, paraffin embedded human lung squamous carcinoma tissue by Immunohistochemistry, using peroxidase conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
References for Anti-Cytokeratin 16 antibody [LL025], prediluted (ab75125)
has not yet been referenced specifically in any publications.
Publishing research using ab75125? Please let us know so that we can cite the reference in this datasheet.
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