Overview

  • Product nameAnti-Cytokeratin 17 antibody
    See all Cytokeratin 17 primary antibodies
  • Description
    Rabbit polyclonal to Cytokeratin 17
  • Specificityab53707 detects endogenous levels of total Cytokeratin 17 protein.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human Cytokeratin 17.

  • Positive control
    • Human breast carcinoma tissue; HuvEc cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab53707 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/20000.

Target

  • FunctionMay play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
  • Tissue specificityExpressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).
  • Involvement in diseaseDefects in KRT17 are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
    Defects in KRT17 are a cause of steatocystoma multiplex (SM) [MIM:184500]. SM is a disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs.
    Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 39.1 antibody
    • CK 17 antibody
    • CK-17 antibody
    • Cytokeratin-17 antibody
    • K17 antibody
    • K1C17_HUMAN antibody
    • Keratin 17 antibody
    • keratin 17 epitope S1 antibody
    • keratin 17 epitope S2 antibody
    • keratin 17 epitope S4 antibody
    • Keratin 17, type I antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 17 antibody
    • keratin, type i cytoskeletal 17 [version 1] antibody
    • Keratin-17 antibody
    • KRT17 antibody
    • PC antibody
    • PC2 antibody
    • PCHC1 antibody
    • type I cytoskeletal 17 antibody
    see all

Anti-Cytokeratin 17 antibody images

  • ab53707 at 1/50 dilution staining Cytokeratin 17 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-Cytokeratin 17 antibody (ab53707) at 1/500 dilution

    Lane 1 : HuvEc cell extract
    Lane 2 : HuvEc cell extract with immunising peptide


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • ICC/IF image of ab53707 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Cytokeratin 17 antibody (ab53707)

This product has been referenced in:
  • Liu Y  et al. Fbxo30 Regulates Mammopoiesis by Targeting the Bipolar Mitotic Kinesin Eg5. Cell Rep 15:1111-22 (2016). IHC-P ; Mouse . Read more (PubMed: 27117404) »
  • Dixon D  et al. A potential mode of action for Anakinra in patients with arthrofibrosis following total knee arthroplasty. Sci Rep 5:16466 (2015). ICC/IF ; Human . Read more (PubMed: 26553966) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (epidermis)
Specification epidermis
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Nov 24 2009

Thank you for your enquiry. I have contacted the originator of the product and it seems that we had a datasheet error. The correct image for this product is not on the datasheet at the moment, and we are currently getting the updated versions from ...

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