Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)




Our Abpromise guarantee covers the use of ab109725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

For unpurified use at 1/100 - 1/250.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionMay play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
    • Tissue specificityExpressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).
    • Involvement in diseaseDefects in KRT17 are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
      Defects in KRT17 are a cause of steatocystoma multiplex (SM) [MIM:184500]. SM is a disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs.
      Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 39.1 antibody
      • CK 17 antibody
      • CK-17 antibody
      • Cytokeratin-17 antibody
      • K17 antibody
      • K1C17_HUMAN antibody
      • Keratin 17 antibody
      • keratin 17 epitope S1 antibody
      • keratin 17 epitope S2 antibody
      • keratin 17 epitope S4 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 17 antibody
      • keratin, type i cytoskeletal 17 [version 1] antibody
      • Keratin-17 antibody
      • KRT17 antibody
      • PC antibody
      • PC2 antibody
      • PCHC1 antibody
      • type I cytoskeletal 17 antibody
      see all

    Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker images

    • All lanes : Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HACAT cell lysates

      Lysates/proteins at 10 µg per lane.

      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 48 kDa
    • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling Cytokeratin 17 with purified ab109725 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Flow Cytometry analysis of A431(human epidermoid carcinoma) cells labeling Cytokeratin 17 with purified ab109725 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    • Immunohistochemical analysis of paraffin-embedded human squamous cervical carcinoma tissue using ab109725 at 1/100 dilution
    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109725 at 1/100 dilution
    • Immunofluorescent staining of A431 cells using ab109725 at 1/100 dilution

    References for Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

    This product has been referenced in:
    • Doucet YS  et al. The touch dome defines an epidermal niche specialized for mechanosensory signaling. Cell Rep 3:1759-65 (2013). ICC/IF ; Mouse . Read more (PubMed: 23727240) »
    • Johnson EK  et al. Identification of new dystroglycan complexes in skeletal muscle. PLoS One 8:e73224 (2013). Mouse . Read more (PubMed: 23951345) »

    See all 3 Publications for this product

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    Thank you for your enquiry regarding ab109725.

    We have uses 0.01M Sodium Citrate Buffer (pH 6.0), as described below:

    To prepare Antigen Retrieval stock solutions:

    - 10X Stock: Dissolve 29.4 g sodium citrate trisodium s...

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