• Product nameAnti-Cytokeratin 18 antibody
    See all Cytokeratin 18 primary antibodies
  • Description
    Rabbit polyclonal to Cytokeratin 18
  • SpecificityKeratin 18 (Ab-33) antibody detects endogenous levels of total Keratin 18 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Keratin 18 around the phosphorylation site of serine 33 (A-A-SP-V-Y).

  • Positive control
    • Human breast carcinoma tissue, extracts from HeLa cells treated with UV light for 5 minutes.


Associated products


Our Abpromise guarantee covers the use of ab51148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.


  • FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody images

  • This image shows human breast carcinoma tissue stained with ab51148 at 1/50 dilution. The cells in the right hand image were treated with the immunogenic peptide, in the left hand image they were not.
  • All lanes : Anti-Cytokeratin 18 antibody (ab51148) at 1/500 dilution

    Lane 1 : HeLa cell extract treated with UV light for 5 minutes, with the immunising peptide
    Lane 2 : HeLa cell extract treated with UV light for 5 minutes, without the immunising peptide

    Predicted band size : 48 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)

References for Anti-Cytokeratin 18 antibody (ab51148)

ab51148 has not yet been referenced specifically in any publications.

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