Anti-Cytokeratin 18 antibody [C-04] (Biotin) (ab27553)


  • Product nameAnti-Cytokeratin 18 antibody [C-04] (Biotin)
    See all Cytokeratin 18 primary antibodies
  • Description
    Mouse monoclonal [C-04] to Cytokeratin 18 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-P, IP, WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: all Mammals
  • Immunogen

    Cytoskeleton preparation of epidermal carcinoma cell line A431.



Our Abpromise guarantee covers the use of ab27553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP 1/500.
WB 1/500. Predicted molecular weight: 47 kDa.
ICC 1/500.
ICC/IF Use at an assay dependent concentration. PubMed: 18556507


  • FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [C-04] (Biotin) images

  • ab27553 at 1/100 staining mouse ES cell derived hepatocyte-like cells by ICC/IF. The cells were formaldehyde fixed and permeabilized with Triton X100 before being blocked with BSA and then incubated with the antibody for 18 hoursat 4°C.

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  • ab27553 staining Cytokeratin 18 in Mouse mammary gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 1:1 Acetone:Methanol and permeabilized with 0.025% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was applied at a concentration of 2 µg/mL and incubated with the sample for 15 hours at 4°C. The secondary antibody was Streptavidin-Cy3®-conjugated Mouse monoclonal, applied at a concentration of 1 µg/mL.

    See Abreview

References for Anti-Cytokeratin 18 antibody [C-04] (Biotin) (ab27553)

This product has been referenced in:
  • Prater MD  et al. Mammary stem cells have myoepithelial cell properties. Nat Cell Biol 16:942-50 (2014). IHC-P ; Mouse . Read more (PubMed: 25173976) »
  • Kim S  et al. FGFR2 promotes breast tumorigenicity through maintenance of breast tumor-initiating cells. PLoS One 8:e51671 (2013). IHC-P ; Mouse . Read more (PubMed: 23300950) »

See all 3 Publications for this product

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This product has been tested in mouse and because of a 97% homology with rat and an 87% homolgy with human it is predicted to work in both species. There is an eight amino acid gap near the N terminus between t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mammary gland)
Specification Mammary gland
Fixative Acetone:methanol (1:1)
Permeabilization Yes - 0.025% Triton X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse ES cell drived hepatocyte-like cells)
Specification mouse ES cell drived hepatocyte-like cells
Fixative Formaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Eugen Kolossov

Verified customer

Submitted Nov 01 2007