Anti-Cytokeratin 18 antibody [C-04] (Biotin) (ab27553)

Overview

  • Product nameAnti-Cytokeratin 18 antibody [C-04] (Biotin)
    See all Cytokeratin 18 primary antibodies
  • Description
    Mouse monoclonal [C-04] to Cytokeratin 18 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-P, IP, WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: all Mammals
  • Immunogen

    Cytoskeleton preparation of epidermal carcinoma cell line A431.

Properties

Applications

Our Abpromise guarantee covers the use of ab27553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP 1/500.
WB 1/500. Predicted molecular weight: 47 kDa.
ICC 1/500.
ICC/IF Use at an assay dependent concentration. PubMed: 18556507

Target

  • FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [C-04] (Biotin) images

  • ab27553 at 1/100 staining mouse ES cell derived hepatocyte-like cells by ICC/IF. The cells were formaldehyde fixed and permeabilized with Triton X100 before being blocked with BSA and then incubated with the antibody for 18 hoursat 4°C.

    See Abreview

  • ab27553 staining Cytokeratin 18 in Mouse mammary gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 1:1 Acetone:Methanol and permeabilized with 0.025% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was applied at a concentration of 2 µg/mL and incubated with the sample for 15 hours at 4°C. The secondary antibody was Streptavidin-Cy3®-conjugated Mouse monoclonal, applied at a concentration of 1 µg/mL.

    See Abreview

References for Anti-Cytokeratin 18 antibody [C-04] (Biotin) (ab27553)

This product has been referenced in:
  • Prater MD  et al. Mammary stem cells have myoepithelial cell properties. Nat Cell Biol 16:942-50 (2014). IHC-P ; Mouse . Read more (PubMed: 25173976) »
  • Kim S  et al. FGFR2 promotes breast tumorigenicity through maintenance of breast tumor-initiating cells. PLoS One 8:e51671 (2013). IHC-P ; Mouse . Read more (PubMed: 23300950) »

See all 3 Publications for this product

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This product has been tested in mouse and because of a 97% homology with rat and an 87% homolgy with human it is predicted to work in both species. There is an eight amino acid gap near the N terminus between t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mammary gland)
Specification Mammary gland
Fixative Acetone:methanol (1:1)
Permeabilization Yes - 0.025% Triton X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse ES cell drived hepatocyte-like cells)
Specification mouse ES cell drived hepatocyte-like cells
Fixative Formaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eugen Kolossov

Verified customer

Submitted Nov 01 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"