• Product nameAnti-Cytokeratin 18 antibody [CY-90]
    See all Cytokeratin 18 primary antibodies
  • Description
    Mouse monoclonal [CY-90] to Cytokeratin 18
  • SpecificityThe antibody reacts specifically with a wide variety of simple epithelia (e.g. intestine, respiratory and urinary systems, liver, and glandular epithelia). It does not react with stratified squamous epithelia (e.g. esophagus or epidermis) or with non-epithelial cells.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human, African Green Monkey
  • Immunogen

    Human epidermal carcinoma (A-431) and MCF-7 human breast cancer cells



Our Abpromise guarantee covers the use of ab49824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/800.
ICC/IF 1/500.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.


  • FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [CY-90] images

  • ab49824 staining Cytokeratin 18 (green) in Human hESC-derived cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with SAP buffer (0.5% saponin, 1% BSA, 0.1% sodium azide) for 30 minutes at room temperature. Cells were blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/800 in SAP buffer) overnight at 4°C. An Alexa Fluor®488-conjugated anti-mouse IgG1 (1/1000) was used as the secondary antibody. Blue - DAPI, Red - albumin

References for Anti-Cytokeratin 18 antibody [CY-90] (ab49824)

This product has been referenced in:
  • Lim YY  et al. Epigenetic modulation of the miR-200 family is associated with transition to a breast cancer stem-cell-like state. J Cell Sci 126:2256-66 (2013). ICC/IF ; Human . Read more (PubMed: 23525011) »
  • Rooper L  et al. Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation. J Ovarian Res 6:57 (2013). Read more (PubMed: 23958497) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (Cos7)
Specification Cos7
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

Dr. clare davy

Verified customer

Submitted May 28 2009