Tissue/ cell preparation (Human). (Breast carcinoma cell line PMC-42).
Our Abpromise guarantee covers the use of ab669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|ELISA||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IHC-P||1/10 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500. Predicted molecular weight: 45 kDa.|
|IP||Use at an assay dependent concentration.|