Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
Key features and details
- Mouse monoclonal [DC 10] to Cytokeratin 18
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 18 antibody [DC 10]
See all Cytokeratin 18 primary antibodies -
Description
Mouse monoclonal [DC 10] to Cytokeratin 18 -
Host species
Mouse -
Specificity
Specific reactivity for the cytokeratin 18 (45 kD) polypeptide. -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Sheep, Hamster, Cow, Dog, Pig -
Immunogen
Tissue, cells or virus corresponding to Human Cytokeratin 18. PMC-42 human breast carcinoma cells.
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Positive control
- ICC/IF: Hep-2 cells. IHC-P: Human colon carcinoma. WB: HeLa, HT-29 cell lysate. Flow Cyt (Intra): MCF-7 cells; HeLa cells.
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General notes
This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS -
Primary antibody notes
This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. -
Clonality
Monoclonal -
Clone number
DC 10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 10 µg/ml.
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ICC/IF | (7) |
1/400.
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Flow Cyt (Intra) |
Use a concentration of 6 - 16 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. |
ICC/IF
1/400. |
Flow Cyt (Intra)
Use a concentration of 6 - 16 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
Tissue specificity
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
Involvement in disease
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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Alternative names
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
Images
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Immunocytochemistry staining of Cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 (ab7797, diluted 1/400), detected with GAM IgG-Alexa Fluor®488 (diluted 1/200; green), cell nuclei stained with PI (1 µg/ml; orange).
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Immunohistochemistry staining of human colon (paraffin sections) using ab7797 10 μg/ml
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All lanes : Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 2 µg/ml
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Non-Reducing)
Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Non-Reducing)
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Reducing)
Lane 4 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Reducing)
Secondary
All lanes : IRDye800-conjugated anti-mouse secondary antibody.A specific band was detected for cytokeratin 18 at approximately 46 kDa, and its proteolytic fragment at approximately 25 kDa.
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Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Overlay histogram showing MCF-7 cells stained with ab7797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7797, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
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Flow cytometry analysis (intracellular staining) of MCF-7 cells (red-filled) stained using ab7797 anti-cytokeratin 18 purified antibody (concentration in sample 0,6 μg/ml, GAM APC). Isotype control: Caco-2 cells (black-dashed).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab7797 has been referenced in 17 publications.
- Wang F et al. MRCKα is a novel regulator of prolactin-induced lactogenesis in bovine mammary epithelial cells. Anim Nutr 10:319-328 (2022). PubMed: 35891685
- Carmell N et al. Identification and Validation of ERK5 as a DNA Damage Modulating Drug Target in Glioblastoma. Cancers (Basel) 13:N/A (2021). PubMed: 33668183
- Han L et al. Conditionally reprogrammed macaque endocervical cells retain steroid receptor expression and produce mucus. Biol Reprod 102:1191-1202 (2020). PubMed: 32232331
- Al-Khalaf HH et al. Senescent Breast Luminal Cells Promote Carcinogenesis through Interleukin-8-Dependent Activation of Stromal Fibroblasts. Mol Cell Biol 39:N/A (2019). PubMed: 30397077
- Deng H et al. Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures. J Cell Physiol 234:7683-7694 (2019). PubMed: 30609028