Overview

  • Product name
    Anti-Cytokeratin 18 antibody [DC 10]
    See all Cytokeratin 18 primary antibodies
  • Description
    Mouse monoclonal [DC 10] to Cytokeratin 18
  • Specificity
    Specific reactivity for the cytokeratin 18 (45 kD) polypeptide.
  • Tested applications
    Suitable for: IHC-Fr, ICC, IHC-P, Flow Cyt, ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Sheep, Hamster, Cow, Dog, Pig, a wide range of other species
  • Immunogen

    PMC-42 human breast carcinoma cells.

  • Positive control
    • IHC-P: Colon carcinoma WB, ELISA, IP: MCF-7 or HeLa cell lysate
  • General notes


    This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
  • Clonality
    Monoclonal
  • Clone number
    DC 10
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/10 - 1/100.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration. (Direct)
WB 1/1000.
IP Use at an assay dependent concentration.

Target

  • Function
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [DC 10] images

  • ab7797 staining human LNCaP cells by ICC/IF.  Cells were acetone fixed and permeabilized in 0.1% Triton-X prior to blocking with 1% fish gelatin for 1 hour at 4°C.  The primary antibody was diluted 1/100 and incubated with the sample for 1 hour.  An Alexa Fluor® conjugated goat anti-mouse antibody, diluted 1/1000, was used as the secondary.

    See Abreview

  • Ab7797 staining cells from a human pancreatic cancer cell line by ICC/IF.  The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C.  Ab7797 was diluted 1/100 and incubated with the sample for 1 hour at 22°C.  An Alexa Fluor® 488 conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview

  • Overlay histogram showing MCF-7 cells stained with ab7797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7797, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

References for Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

This product has been referenced in:
  • Cadieux C  et al. Mouse mammary tumor virus p75 and p110 CUX1 transgenic mice develop mammary tumors of various histologic types. Cancer Res 69:7188-97 (2009). WB ; Mouse . Read more (PubMed: 19738070) »
  • Letellier G  et al. Epithelial phenotypes in the developing human prostate. J Histochem Cytochem 55:885-90 (2007). IHC-P ; Human . Read more (PubMed: 17478449) »

See all 5 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human retinal pigment epithelium (ARPE-19))
Permeabilization
Yes - TritonX 0,3%
Specification
Human retinal pigment epithelium (ARPE-19)
Blocking step
Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Jun 26 2015

Application
ELISpot
Sample
Human Recombinant protein (E coli)
Specification
E coli
Username

Abcam user community

Verified customer

Submitted May 12 2015

Application
ELISA
Sample
Human Recombinant protein (Human Cytokeratin 18 full length protein (ab73638))
Specification
Human Cytokeratin 18 full length protein (ab73638)
Type
Sandwich (Detection)
Blocking step
StartingBlock blocking buffer (Thermo Scientific) as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

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Submitted Feb 25 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (hIPSC-derived endocrine cells)
Specification
hIPSC-derived endocrine cells
Permeabilization
Yes - 0.1 % triton x100
Fixative
Formaldehyde
Username

Dr. Youngjin Kim

Verified customer

Submitted Mar 24 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human breast carcinoma xenograft in mouse)
Specification
Human breast carcinoma xenograft in mouse
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
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Submitted May 01 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA468)
Specification
MDA468
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted May 01 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (C4-2b (prostate cancer cell line))
Specification
C4-2b (prostate cancer cell line)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 0.2% · Temperature: 21°C
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Verified customer

Submitted Jul 15 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (pancreatic cancer cell line)
Loading amount
20 µg
Specification
pancreatic cancer cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Hannah Whiteman

Verified customer

Submitted Jul 04 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (pancreatic cancer cell line)
Specification
pancreatic cancer cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Hannah Whiteman

Verified customer

Submitted Jun 18 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP)
Specification
LNCaP
Fixative
Acetone
Permeabilization
Yes - 0.1% Triton-X
Blocking step
Fish Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Vivien D'Arcy

Verified customer

Submitted Mar 06 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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