Anti-Cytokeratin 18 antibody [DC-10] (Biotin) (ab27551)

Overview

  • Product name
    Anti-Cytokeratin 18 antibody [DC-10] (Biotin)
    See all Cytokeratin 18 primary antibodies
  • Description
    Mouse monoclonal [DC-10] to Cytokeratin 18 (Biotin)
  • Conjugation
    Biotin
  • Specificity
    ab27551 recognises cytokeratin peptide 18 (45 kDa) in human tissue.
  • Tested applications
    Suitable for: IHC-P, IP, WB, ICC, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human) - human breast carcinoma cell line PMC-42.

  • Positive control
    • HeLa human cervix carcinoma cell, MCF-7 human breast adenocarcinoma cell

Properties

Applications

Our Abpromise guarantee covers the use of ab27551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB 1/500. Predicted molecular weight: 47 kDa.
ICC Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Images

  • Flow Cytometry analysis of HeLa cells labeling Cytokeratin 18 with ab27551.

  • Anti-Cytokeratin 18 antibody [DC-10] (Biotin) (ab27551) (1-2 ug/ml dilution) + HeLa cell lysate

    Performed under non-reducing conditions.

    Predicted band size : 47 kDa

References

ab27551 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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