Anti-Cytokeratin 18 antibody [SP69], prediluted (ab101542)

Overview

  • Product nameAnti-Cytokeratin 18 antibody [SP69], prediluted
    See all Cytokeratin 18 primary antibodies
  • Description
    Rabbit monoclonal [SP69] to Cytokeratin 18, prediluted
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to the C terminus of Human Cytokeratin 18.

  • Positive control
    • Human breast carcinomaand Human prostate carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab101542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. (Prediluted). Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.

Target

  • FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Anti-Cytokeratin 18 antibody [SP69], prediluted images

  • ab101542, prediluted, staining Cytokeratin 18 in formalin fixed, paraffin embedded Human breast carcinoma by Immunohistochemistry.
  • Anti-Cytokeratin 18 antibody [SP69] (ab93741) at 1/100 dilution + MCF-7 cell lysate

    Predicted band size : 48 kDa

References for Anti-Cytokeratin 18 antibody [SP69], prediluted (ab101542)

ab101542 has not yet been referenced specifically in any publications.

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