Anti-Cytokeratin 19 antibody [A53-B/A2] (ab7754)

Overview

  • Product nameAnti-Cytokeratin 19 antibody [A53-B/A2]
    See all Cytokeratin 19 primary antibodies
  • Description
    Mouse monoclonal [A53-B/A2] to Cytokeratin 19
  • SpecificityRod domain of cytokeratin peptide 19 (40 kDa) in human tissue.
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, Flow Cyt, WB, IHC-P, ICC, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human mammary carcinoma cell line MCF-7.

  • EpitopeRod domain of cytokeratin peptide 19.
  • Positive control
    • This antibody gave a positive result in IF/ICC when used in the following methanol fixed cell lines: HepG2.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites using protein A-affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberA53-B/A2
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21339324
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • FunctionInvolved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificityExpressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stagePresent in hair follicles at all stages of development.
  • DomainThis keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40 kDa keratin intermediate filament antibody
    • CK 19 antibody
    • CK-19 antibody
    • CK19 antibody
    • Cytokeratin 19 antibody
    • Cytokeratin-19 antibody
    • K19 antibody
    • K1C19_HUMAN antibody
    • K1CS antibody
    • Keratin 19 antibody
    • Keratin type I 40 kD antibody
    • Keratin type I 40kD antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin, type I cytoskeletal 19 antibody
    • Keratin, type I, 40 kd antibody
    • Keratin-19 antibody
    • KRT19 antibody
    • MGC15366 antibody
    see all

Anti-Cytokeratin 19 antibody [A53-B/A2] images

  • All lanes : Anti-Cytokeratin 19 antibody [A53-B/A2] (ab7754) at 1/1000 dilution

    Lane 1 : Lung tumour lysate (patient 1)
    Lane 2 : Lung tumour lysate (patient 2)
    Lane 3 : Lung tumour lysate (patient 3)
    Lane 4 : Lung tumour lysate (patient 4)

    Lysates/proteins at 5 µg per lane.

    Secondary
    Goat anti mouse HRP (H and L) at 1/40000 dilution

    Performed under reducing conditions.

    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

    See Abreview

  • ab7754 staining Cytokeratin 19 in human skin.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab7754 staining Cytokeratin 19 in Human liver tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 30 minutes at 37°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in antibody diluent) for 1 hour at 37°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing MCF7 cells stained with ab7754 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7754, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunofluorescence analysis of 1 month hepato-differentiated Human dental pulp stem cells, staining Cytokeratin 19 with ab7754 at 1/60 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.
  • ICC/IF image of ab7754 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7754 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Cytokeratin 19 antibody [A53-B/A2] (ab7754)

This product has been referenced in:
  • Ingthorsson S  et al. HER2 induced EMT and tumorigenicity in breast epithelial progenitor cells is inhibited by coexpression of EGFR. Oncogene N/A:N/A (2015). WB, IHC ; Human . Read more (PubMed: 26686087) »
  • Roy I  et al. CXCL12 chemokine expression suppresses human pancreatic cancer growth and metastasis. PLoS One 9:e90400 (2014). IHC-P ; Human . Read more (PubMed: 24594697) »

See all 14 Publications for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Tissue lysate - whole (colon mucosa)
Specification colon mucosa
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Apr 14 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample Human Tissue sections (human colon carcinoma)
Specification human colon carcinoma
Permeabilization No
Fixative Acetone
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Abcam user community

Verified customer

Submitted Nov 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS for 3 min
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 100 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (10% Tris-glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Sep 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Blocking step PBS + 0.1% Triton X-100, 1% BSA, 10% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Verified customer

Submitted Aug 03 2012

Thank you for contacting us and for your interest in our products. I am sorry to confirm that as far as we are aware, ab7754 has never been tested with samples from mouse. All tested and guaranteed species cross-reactivity information is stated on our...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (liver)
Specification liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Jun 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (lung tumor lysate)
Specification lung tumor lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Mike Campa

Verified customer

Submitted Oct 19 2005

We regret to inform you that the epitope of these antibodies have yet not been mapped.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"