Overview

  • Product nameAnti-Cytokeratin 19 antibody
    See all Cytokeratin 19 primary antibodies
  • Description
    Rabbit polyclonal to Cytokeratin 19
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Cytokeratin 19.

    (Peptide available as ab94822.)

  • Positive control
    • This antibody gave a positive signal in Human skin tissue lysate and the following whole cell lysates: HepG2; MDA-MB-361

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • Tissue specificityExpressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Developmental stagePresent in hair follicles at all stages of development.
    • DomainThis keratin differs from all other IF proteins in lacking the C-terminal tail domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • 40 kDa keratin intermediate filament antibody
      • CK 19 antibody
      • CK-19 antibody
      • CK19 antibody
      • Cytokeratin 19 antibody
      • Cytokeratin-19 antibody
      • K19 antibody
      • K1C19_HUMAN antibody
      • K1CS antibody
      • Keratin 19 antibody
      • Keratin type I 40 kD antibody
      • Keratin type I 40kD antibody
      • Keratin type I cytoskeletal 19 antibody
      • Keratin, type I cytoskeletal 19 antibody
      • Keratin, type I, 40 kd antibody
      • Keratin-19 antibody
      • KRT19 antibody
      • MGC15366 antibody
      see all

    Anti-Cytokeratin 19 antibody images

    • All lanes : Anti-Cytokeratin 19 antibody (ab85351) at 1 µg/ml

      Lane 1 : Human skin tissue lysate - total protein (ab30166)
      Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 44 kDa
      Observed band size : 44 kDa
      Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute
    • Anti-Cytokeratin 19 antibody (ab85351) at 1 µg/ml + Recombinant Human Cytokeratin 19 protein (ab73639) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

    References for Anti-Cytokeratin 19 antibody (ab85351)

    ab85351 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab85351.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"