• Product nameAnti-Cytokeratin 19 antibody [EP1580Y]
    See all Cytokeratin 19 primary antibodies
  • Description
    Rabbit monoclonal [EP1580Y] to Cytokeratin 19
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cytokeratin 19 (C terminal).

  • Positive control
    • WB: HepG2 and NIH/3T3 cell lysates. IHC-P: Human skin, breast carcinoma, kidney carcinoma, endometrial carcinoma and gastric adenocarcinoma tissues. ICC/IF: HepG2 and MCF-7 cells. Flow Cyt: MCF-7 and HeLa cells.
  • General notes


    A trial size is available to purchase for this antibody.


    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab52625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/500.

For unpurified, use 1/50.

IHC-Fr Use at an assay dependent concentration.
WB 1/50000 - 1/200000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

For unpurified, use 1/10000 - 1/50000.

Flow Cyt 1/30 - 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/400 - 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified, use at 1/100.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionInvolved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • Tissue specificityExpressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Developmental stagePresent in hair follicles at all stages of development.
    • DomainThis keratin differs from all other IF proteins in lacking the C-terminal tail domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • 40 kDa keratin intermediate filament antibody
      • CK 19 antibody
      • CK-19 antibody
      • CK19 antibody
      • Cytokeratin 19 antibody
      • Cytokeratin-19 antibody
      • K19 antibody
      • K1C19_HUMAN antibody
      • K1CS antibody
      • Keratin 19 antibody
      • Keratin type I 40 kD antibody
      • Keratin type I 40kD antibody
      • Keratin type I cytoskeletal 19 antibody
      • Keratin, type I cytoskeletal 19 antibody
      • Keratin, type I, 40 kd antibody
      • Keratin-19 antibody
      • KRT19 antibody
      • MGC15366 antibody
      see all

    Anti-Cytokeratin 19 antibody [EP1580Y] images

    • Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) cells labelling Cytokeratin 19 (green) with purified ab52625 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291, anti-Tubulin (mouse mAb) at 1/1000 followed by ab150120 AlexaFluor®594 goat anti-mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

      For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by anti-rabbit secondary antibody (ab150077).  

    • All lanes : Anti-Cytokeratin 19 antibody [EP1580Y] (ab52625) at 1/45000 dilution (purified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : NIH/3T3 cell lysate

      Lysates/proteins at 20 µg per lane.

      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 44 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin-embedded human skin with purified ab52625 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • ab52625 staining Cytokeratin 19 in the human cell line MCF-7 (human breast carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • Immunofluorescent staining of MCF-7 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab52625 at a dilution of 1/200. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200 and the cells were counter stained with DAPI.

    • Unpurified ab52625 showing positive staining in Breast carcinoma tissue.

    • Overlay histogram showing HeLa cells stained with unpurified ab52625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52625, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Unpurified ab52625 showing positive staining in Endometrial carcinoma tissue.

    • Unpurified ab52625 showing negative staining in Glioma tissue.

    • Unpurified ab52625 showing positive staining in Gastric adenocarcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Cytokeratin 19 antibody [EP1580Y] (ab52625)

    This product has been referenced in:
    • Kim JY  et al. Apamin suppresses biliary fibrosis and activation of hepatic stellate cells. Int J Mol Med 39:1188-1194 (2017). IHC-P ; Mouse . Read more (PubMed: 28405682) »
    • Feng F  et al. Guidance to rational use of pharmaceuticals in gallbladder sarcomatoid carcinoma using patient-derived cancer cells and whole exome sequencing. Oncotarget 8:5349-5360 (2017). IHC ; Human . Read more (PubMed: 28029662) »

    See all 23 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Mouse salivary gland)
    Permeabilization Yes - 0.1% Triton
    Specification Mouse salivary gland
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
    Fixative Formaldehyde

    Dr. Isabelle Miletich

    Verified customer

    Submitted Mar 07 2017

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (tongue)
    Antigen retrieval step Heat mediated
    Permeabilization No
    Specification tongue
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    Submitted Aug 26 2016

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample Human Cell (HCC1143 cell lines)
    Specification HCC1143 cell lines
    Permeabilization Yes - 0.3% Triton X-100 in TBS
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 25 2015

    Application Flow Cytometry
    Fixation Paraformaldehyde
    Permeabilization Yes - 0.2% Triton X-100 in PBS
    Sample Human Cell (CK19 positive and negative cell lines)
    Specification CK19 positive and negative cell lines
    Gating Strategy live cells, doubled were excluded based on FSC - SSC profile
    Preparation Cell harvesting/tissue preparation method: Cells were harvested with trypsin and fixed with 2% PFA / PBS for 15 min on ice. Following cell fixation, PFA was removed, cells were washed with ice-cold PBS, permeabilised and blocked with 0.2% Triton X-100 / 5% FBS / PBS for 30 min on ice prior to staining
    Sample buffer: PBS

    Abcam user community

    Verified customer

    Submitted Feb 05 2015

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (4-12)
    Sample Human Cell lysate - whole cell (panel of breast cancer cell lines)
    Specification panel of breast cancer cell lines
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Dec 18 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Liver)
    Specification Liver
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat pH 6,0
    Permeabilization Yes - 0,5% Tween 20
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mrs. Sabine Pohl

    Verified customer

    Submitted Jul 14 2008