Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)


  • Product nameAnti-Cytokeratin 19 antibody [EPR1579Y]
    See all Cytokeratin 19 primary antibodies
  • Description
    Rabbit monoclonal [EPR1579Y] to Cytokeratin 19
  • SpecificityIn our WB testing this antibody did not recognize mouse Cytokeratin 19, but customers have reported the antibody recognizes the protein in IHC-P with mouse tissue. Thus, we have removed mouse as a "does not react with" species and welcome further feedback from other researchers using this antibody in mouse.
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cytokeratin 19 (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: T47D, HepG2 or SKBR-3 cell lysate; IHC-P: Human stomach adenocarcinoma tissue. IF/ICC: HCT116 cell line.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab76539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 44 kDa.
IP 1/20.
Flow Cyt 1/20. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.


  • FunctionInvolved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificityExpressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stagePresent in hair follicles at all stages of development.
  • DomainThis keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40 kDa keratin intermediate filament antibody
    • CK 19 antibody
    • CK-19 antibody
    • CK19 antibody
    • Cytokeratin 19 antibody
    • Cytokeratin-19 antibody
    • K19 antibody
    • K1C19_HUMAN antibody
    • K1CS antibody
    • Keratin 19 antibody
    • Keratin type I 40 kD antibody
    • Keratin type I 40kD antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin, type I cytoskeletal 19 antibody
    • Keratin, type I, 40 kd antibody
    • Keratin-19 antibody
    • KRT19 antibody
    • MGC15366 antibody
    see all

Anti-Cytokeratin 19 antibody [EPR1579Y] images

  • Immunofluorescence staining of MCF-7 cells with purified ab76539 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • All lanes : Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539) at 1/10000 dilution

    Lane 1 : T47D cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : SKBR-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 44 kDa
  • Immunohistochemical staining of human stomach adenocarcinoma tissue with 1/100-1/250 ab76539.
  • ab76539 stained HCT116 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76539 at 1/200 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab76539. Green- CK19 red-PI.

  • Overlay histogram showing MCF7 cells stained with ab76539 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76539, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

This product has been referenced in:
  • Aliagas E  et al. High expression of ecto-nucleotidases CD39 and CD73 in human endometrial tumors. Mediators Inflamm 2014:509027 (2014). IHC . Read more (PubMed: 24707115) »
  • Galvão AM  et al. Immune-endocrine cross-talk in reproductive biology and pathology. Mediators Inflamm 2014:856465 (2014). IHC ; Human . Read more (PubMed: 24872600) »

See all 3 Publications for this product

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Although mouse samples were not recommended based on the western blot results below with these antibodies, there is still the possibility that the negative results in mouse might be due to the limited sample types, especially for the antibodies with hi...

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Thank you very much for contacting us. Following your request, I have searched our catalog for all antibodies that match the mentioned criteria, i.e. that recognise human targets and that do not react with mouse. Please find attached a list of ...

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