This antibody stains practically all epithelial tumours, especially adenocarcinoma. Staining is negative in hepatocellular carcinoma and also in most medullary and weakly differentiated ductal carcinomas.
Storage instructionsShipped at 4°C. Store at +4°C.
Primary antibody notesThis antibody stains practically all epithelial tumours, especially adenocarcinoma. Staining is negative in hepatocellular carcinoma and also in most medullary and weakly differentiated ductal carcinomas.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use Neat, 100µl per test.
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 48 kDa).
FunctionInvolved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityExpressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stagePresent in hair follicles at all stages of development.
DomainThis keratin differs from all other IF proteins in lacking the C-terminal tail domain.