Anti-Cytokeratin 19 antibody [SB39g] (FITC) (ab87014)

Overview

  • Product nameAnti-Cytokeratin 19 antibody [SB39g] (FITC)
    See all Cytokeratin 19 primary antibodies
  • Description
    Mouse monoclonal [SB39g] to Cytokeratin 19 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: WB, ELISA, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • FC: HT29 cells ELISA and IHC: HT29 and HCC38 cells WB: HT29 and HCC38 cell lysates
  • General notes


    Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesMonoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
  • ClonalityMonoclonal
  • Clone numberSB39g
  • IsotypeIgG2a
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab87014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 10 µg/ml.

Target

  • FunctionInvolved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificityExpressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stagePresent in hair follicles at all stages of development.
  • DomainThis keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40 kDa keratin intermediate filament antibody
    • CK 19 antibody
    • CK-19 antibody
    • CK19 antibody
    • Cytokeratin 19 antibody
    • Cytokeratin-19 antibody
    • K19 antibody
    • K1C19_HUMAN antibody
    • K1CS antibody
    • Keratin 19 antibody
    • Keratin type I 40 kD antibody
    • Keratin type I 40kD antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin, type I cytoskeletal 19 antibody
    • Keratin, type I, 40 kd antibody
    • Keratin-19 antibody
    • KRT19 antibody
    • MGC15366 antibody
    see all

Anti-Cytokeratin 19 antibody [SB39g] (FITC) images

  • Overlay histogram showing MCF-7 cells stained with ab87014 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87014, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a (FITC) (1µg/1x106 cells) for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Cytokeratin 19 antibody [SB39g] (FITC) (ab87014)

ab87014 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience while we checked the information on ab8704.   After discussing this with the lab, it was agreed that ab8704 should have very little chance to cross react to mouse tissue because the antibody came from mouse. The mouse immune...

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The lab has just told me that they will hopefully be able to provide the immunogen information about ab87014 next week. They are tracking down the information and should get back to me soon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"