Anti-Cytokeratin 19 antibody [SB39g] (FITC) (ab87014)

Overview

  • Product name
    Anti-Cytokeratin 19 antibody [SB39g] (FITC)
    See all Cytokeratin 19 primary antibodies
  • Description
    Mouse monoclonal [SB39g] to Cytokeratin 19 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: WB, ELISA, Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • FC: HT29 cells ELISA and IHC: HT29 and HCC38 cells WB: HT29 and HCC38 cell lysates
  • General notes


    Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
  • Clonality
    Monoclonal
  • Clone number
    SB39g
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.

Target

  • Function
    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity
    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Developmental stage
    Present in hair follicles at all stages of development.
  • Domain
    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40 kDa keratin intermediate filament antibody
    • CK 19 antibody
    • CK-19 antibody
    • CK19 antibody
    • Cytokeratin 19 antibody
    • Cytokeratin-19 antibody
    • K19 antibody
    • K1C19_HUMAN antibody
    • K1CS antibody
    • Keratin 19 antibody
    • Keratin type I 40 kD antibody
    • Keratin type I 40kD antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin, type I cytoskeletal 19 antibody
    • Keratin, type I, 40 kd antibody
    • Keratin-19 antibody
    • KRT19 antibody
    • MGC15366 antibody
    see all

Images

  • Overlay histogram showing MCF-7 cells stained with ab87014 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87014, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a (FITC) (1µg/1x106 cells) for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Ab87014 staining Cytokeratin 19 in MIA PaCa-2 cells ( Human pancreatic carcinoma cell line) .

References

This product has been referenced in:
  • Santoro A  et al. Relationship between CK19 expression, deregulation of normal keratinocyte differentiation pattern and high risk-human papilloma virus infection in oral and oropharyngeal squamous cell carcinoma. Infect Agent Cancer 10:46 (2015). Flow Cyt . Read more (PubMed: 26672675) »
  • Cardinale V  et al. Profiles of cancer stem cell subpopulations in cholangiocarcinomas. Am J Pathol 185:1724-39 (2015). Read more (PubMed: 25892683) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for your patience while we checked the information on ab8704.   After discussing this with the lab, it was agreed that ab8704 should have very little chance to cross react to mouse tissue because the antibody came from mouse. The mouse immune...

Read More

The lab has just told me that they will hopefully be able to provide the immunogen information about ab87014 next week. They are tracking down the information and should get back to me soon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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