The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesElectron Microscopy: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
WB: 1/1000 - 1/3000. Use BSA (instead of milk, which contains some keratins) to block nonspecific binding to immunoblots. Predicted molecular weight: 56-70 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProbably contributes to terminal cornification.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageSynthesized during maturation of epidermal keratinocytes.