Overview

  • Product nameAnti-Cytokeratin 20 antibody
    See all Cytokeratin 20 primary antibodies
  • Description
    Rabbit polyclonal to Cytokeratin 20
  • Specificityab53120 detects endogenous levels of total Cytokeratin 20 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    A synthetic peptide derived from human Cytokeratin 20.

  • Positive control
    • HeLa cell extracts and human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab53120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • FunctionPlays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
  • Tissue specificityExpressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageFirst detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
  • Post-translational
    modifications
    Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD20 antibody
    • CK 20 antibody
    • CK-20 antibody
    • CK20 antibody
    • Cytokeratin-20 antibody
    • Cytokeratin20 antibody
    • K1C20_HUMAN antibody
    • K20 antibody
    • KA20 antibody
    • Keratin 20 antibody
    • keratin 20, type I antibody
    • keratin 21, rat, homolog of antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 20 antibody
    • Keratin-20 antibody
    • Keratin20 antibody
    • KRT 20 antibody
    • KRT 21 antibody
    • KRT20 antibody
    • KRT21 antibody
    • MGC35423 antibody
    • OTTHUMP00000164518 antibody
    • Protein IT antibody
    • type I cytoskeletal 20 antibody
    see all

Anti-Cytokeratin 20 antibody images

  • All lanes : Anti-Cytokeratin 20 antibody (ab53120) at 1/300 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • ab53120 at 1/50 dilution staining Cytokeratin 20 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-Cytokeratin 20 antibody (ab53120)

This product has been referenced in:
  • Bhusari S  et al. Mutation Spectra of Kras and Tp53 in Urethral and Lung Neoplasms in B6C3F1 Mice Treated with 3,3',4,4'-Tetrachloroazobenzene. Toxicol Pathol N/A:N/A (2013). Mouse . Read more (PubMed: 23703846) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"