Anti-Cytokeratin 20 antibody (ab53120)

Overview

• Product name
  Anti-Cytokeratin 20 antibody
  See all Cytokeratin 20 primary antibodies
• Description
  Rabbit polyclonal to Cytokeratin 20
• Specificity
  ab53120 detects endogenous levels of total Cytokeratin 20 protein.
• Tested applications
  Suitable for: WB, IHC-P, ELISAmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Rat
• Immunogen

  A synthetic peptide derived from human Cytokeratin 20.

• Positive control
  • HeLa cell extracts and human breast carcinoma tissue.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Intermediate Filaments
  • Class I
  • Cytokeratins

  • Cancer
  • Tumor biomarkers
  • Other

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
• Tissue specificity
  Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
• Sequence similarities
  Belongs to the intermediate filament family.
• Developmental stage
  First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
• Post-translational
  modifications
  Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
  Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
• Cellular localization
  Cytoplasm.

• Target information above from: UniProt accession P35900 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 54474 Human
  • Entrez Gene: 286912 Rat
  • Omim: 608218 Human
  • SwissProt: P35900 Human
  • SwissProt: P25030 Rat
  • Unigene: 84905 Human
  • Unigene: 9887 Rat
• Alternative names
  • CD20 antibody
  • CK 20 antibody
  • CK-20 antibody

  • CK20 antibody
  • Cytokeratin-20 antibody
  • Cytokeratin20 antibody
  • K1C20_HUMAN antibody
  • K20 antibody
  • KA20 antibody
  • Keratin 20 antibody
  • keratin 20, type I antibody
  • keratin 21, rat, homolog of antibody
  • Keratin antibody
  • Keratin type I cytoskeletal 20 antibody
  • Keratin-20 antibody
  • Keratin20 antibody
  • KRT 20 antibody
  • KRT 21 antibody
  • KRT20 antibody
  • KRT21 antibody
  • MGC35423 antibody
  • OTTHUMP00000164518 antibody
  • Protein IT antibody
  • type I cytoskeletal 20 antibody

  see all

Protocols

• Western blot protocols
• Immunohistochemistry protocols