Anti-Cytokeratin 20 antibody - Cytoskeleton Marker (ab97511)
Key features and details
- Rabbit polyclonal to Cytokeratin 20 - Cytoskeleton Marker
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cytokeratin 20 antibody - Cytoskeleton Marker
See all Cytokeratin 20 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 20 - Cytoskeleton Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 75-287 of Human Cytokeratin 20 (NP_061883).
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Positive control
- HeLa cell, HT29 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. -
Tissue specificity
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. -
Post-translational
modificationsHyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 54474 Human
- Entrez Gene: 66809 Mouse
- Omim: 608218 Human
- SwissProt: P35900 Human
- SwissProt: Q9D312 Mouse
- Unigene: 84905 Human
- Unigene: 28042 Mouse
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Alternative names
- CD20 antibody
- CK 20 antibody
- CK-20 antibody
see all
Images
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Ab97511 staining Cytokeratin 20 in Mouse urinary bladder by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Sample was incubated with primary antibody at a 1:500 dilution.
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Anti-Cytokeratin 20 antibody - Cytoskeleton Marker (ab97511) at 1/3000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE -
Ab97511 staining Cytokeratin 20 in HT29 xenograft by Immunohistochemistry (IHC-P - paraformaldehyde - fixed, paraffin embedded sections). Samples were incubated with primary antibody at 1:100 dilution.
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Immunofluorescence analysis of Cytokeratin 20 in paraformaldehyde fixed HeLa, using ab97511 at a 1/200 dilution.
Lower image (2): merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab97511 has been referenced in 6 publications.
- Rahrmann EP et al. The NALCN channel regulates metastasis and nonmalignant cell dissemination. Nat Genet 54:1827-1838 (2022). PubMed: 36175792
- Li S et al. An integrated map of fibroblastic populations in human colon mucosa and cancer tissues. Commun Biol 5:1326 (2022). PubMed: 36463319
- Mastri M et al. Patient derived models of bladder cancer enrich the signal of the tumor cell transcriptome facilitating the analysis of the tumor cell compartment. Am J Clin Exp Urol 9:416-434 (2021). PubMed: 34993263
- Seidlitz T et al. Mouse Models of Human Gastric Cancer Subtypes With Stomach-Specific CreERT2-Mediated Pathway Alterations. Gastroenterology 157:1599-1614.e2 (2019). PubMed: 31585123
- Fasano M et al. Small bowel metastasis from pancreatic cancer in a long-term survival patient with synchronous advanced malignant pleural mesothelioma: A case report and literature review. Oncol Lett 12:4505-4509 (2016). IHC-P ; Human . PubMed: 28105159
- Liu H et al. Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells. Cell Death Dis 4:e857 (2013). IHC-P ; Human . PubMed: 24136221