Recombinant Anti-Cytokeratin 20 antibody [EPR1621(2)] - Cytoskeleton Marker (ab109111)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1621(2)] to Cytokeratin 20 - Cytoskeleton Marker
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 20 antibody [EPR1621(2)] - Cytoskeleton Marker
See all Cytokeratin 20 primary antibodies -
Description
Rabbit monoclonal [EPR1621(2)] to Cytokeratin 20 - Cytoskeleton Marker -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt:: HT-29, SW480; ICC/IF: HT-29 cells; WB: HT-29 and SW480.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1621(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20.
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ICC/IF |
1/50 - 1/100.
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WB |
1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa).
For unpurified use at 1/10000 - 1/50000. |
Notes |
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Flow Cyt (Intra)
1/20. |
ICC/IF
1/50 - 1/100. |
WB
1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa). For unpurified use at 1/10000 - 1/50000. |
Target
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Function
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. -
Tissue specificity
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. -
Post-translational
modificationsHyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 54474 Human
- Omim: 608218 Human
- SwissProt: P35900 Human
- Unigene: 84905 Human
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Alternative names
- CD20 antibody
- CK 20 antibody
- CK-20 antibody
see all
Images
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Flow Cytometry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labelling Cytokeratin 20 with Purified ab109111 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Cytokeratin 20 with Purified ab109111 at 1:50 dilution (3.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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All lanes : Anti-Cytokeratin 20 antibody [EPR1621(2)] - Cytoskeleton Marker (ab109111) at 1/10000 dilution
Lane 1 : HT-29 cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.This data was generated with the unpurified version of this antibody.
False colour image of Western blot:Anti-Cytokeratin 20 antibody [EPR1621(2)] - Cytoskeleton Marker staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109111 was shown to bind specifically to Cytokeratin 20. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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This data was generated with the unpurified version of this antibody.
Intracellular Flow Cytometry analysis of SW480 (human colorectal adenocarcinoma) cells labeling Cytokeratin 20 with unpurified ab109111 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab109111 has not yet been referenced specifically in any publications.