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Rabbit polyclonal to Cytokeratin 2e
Recombinant fragment, corresponding to a region within amino acids 143 - 499 of Human Cytokeratin 2e (NP_000414).
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 65 kDa.
Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner
CK 2e antibody
Epithelial keratin-2e antibody
Western blot - Anti-Cytokeratin 2e antibody (ab96145)
Anti-Cytokeratin 2e antibody (ab96145) at 1/500 dilution + A431 whole cell lysate
at 30 µg Predicted band size: 65 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody (ab96145)
ab96145, at a 1/100 dilution, staining Cytokeratin 2e in paraffin embedded H661 by Immunohistochemical analysis.
has not yet been referenced specifically in any publications.
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