Anti-Cytokeratin 2e antibody [Ks2.342.7.1] (ab19122)

Overview

  • Product nameAnti-Cytokeratin 2e antibody [Ks2.342.7.1]
    See all Cytokeratin 2e primary antibodies
  • Description
    Mouse monoclonal [Ks2.342.7.1] to Cytokeratin 2e
  • SpecificityThis antibody is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with cytokeratin 10). It is also reactive on hyperkeratosis of diverse viral and genetic origin. The antibody does not react with palate cytokeratin 2p.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide:

    SCQISCKSRG RGGGGGGFRG FS

    , corresponding to N terminal amino acids 2 - 23 of Human Keratin 2e.

  • Positive control
    • IHC-P: FFPE human skin tissue sections.
  • General notes


    This antibody represents an excellent marker to study terminal epidermal differentiation.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferConstituent: Tissue culture supernatant
  • PurityTissue culture supernatant
  • Primary antibody notesThis antibody represents an excellent marker to study terminal epidermal differentiation.
  • ClonalityMonoclonal
  • Clone numberKs2.342.7.1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/20.

Target

  • RelevanceKeratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • CK 2e antibody
    • Cytokeratin-2e antibody
    • Epithelial keratin-2e antibody
    • K2e antibody
    • Keratin type II cytoskeletal 2 epidermal antibody
    • Keratin-2 epidermis antibody
    • KRT2 antibody
    • KRT2A antibody
    • KRT2E antibody
    • Type-II keratin Kb2 antibody
    see all

Anti-Cytokeratin 2e antibody [Ks2.342.7.1] images

  • IHC image of Cytokeratin 2e staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19122, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Cytokeratin 2e antibody [Ks2.342.7.1] (ab19122)

ab19122 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"