Overview

  • Product name
    Anti-Cytokeratin 3 antibody [AE5]
    See all Cytokeratin 3 primary antibodies
  • Description
    Mouse monoclonal [AE5] to Cytokeratin 3
  • Host species
    Mouse
  • Specificity
    This antibody recognizes the 64kD polypeptide (cytokeratin 3) and 55 kDa polypeptide (cytokeratin 12) of rabbit corneal epithelium. It also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. The clone number has been updated from (2Q1040) to (AE5) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Cow, Human
  • Immunogen

    Tissue, cells or virus corresponding to Rabbit Cytokeratin 3. Rabbit corneal epithelial keratin.

Properties

Applications

Our Abpromise guarantee covers the use of ab68260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 54 , 65 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50.

Target

  • Tissue specificity
    Cornea specific.
  • Involvement in disease
    Defects in KRT3 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated as MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 65 kDa cytokeratin antibody
    • CK-3 antibody
    • CK3 antibody
    • Cytokeratin-3 antibody
    • K2C3_HUMAN antibody
    • K3 antibody
    • Keratin 3 antibody
    • Keratin antibody
    • Keratin type II cytoskeletal 3 antibody
    • Keratin-3 antibody
    • KRT3 antibody
    • type II cytoskeletal 3 antibody
    • Type-II keratin Kb3 antibody
    see all

Images

  • Immunofluorescence analysis of rabbit cornea cells, staining Cytokeratin 3+12 with ab68620.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/50 in 3% BSA in PBST (0.5% Tween 20)) for 12 hours at 4°C. An AlexaFluor®594-conjugated goat anti-mouse polyclonal IgG (1/100) was used as the secondary antibody.

    See Abreview

  • Immunofluorescent analysis of human ocular surface squamous cell carcinoma tissue, labeling Cytokeratin 3+12 with ab68260. Samples were fixed in 4% paraformaldehyde and blocked wuth 4% BSA with 0.1% Triton X-100. Incubation with ab68260 (diluted 1/50) overnight at 4°C. Samples were then incubated with an Alexa Fluor 488-conjugated secondary antibody for 1 hour, and counterstained with DAPI to visualize nuclei. 

References

This product has been referenced in:

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Cornea)
Specification
Cornea
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Aaron Gardner

Verified customer

Submitted Nov 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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