Overview

  • Product nameAnti-Cytokeratin 3 antibody [AE5]
    See all Cytokeratin 3 primary antibodies
  • Description
    Mouse monoclonal [AE5] to Cytokeratin 3
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rabbit, Cow, Human
  • Immunogen

    Rabbit corneal epithelial keratins

Properties

Applications

Our Abpromise guarantee covers the use of ab77869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/3000. Predicted molecular weight: 65 kDa.
Flow Cyt Use 2µg for 106 cells.

Target

  • Tissue specificityCornea specific.
  • Involvement in diseaseDefects in KRT3 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated as MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 65 kDa cytokeratin antibody
    • CK-3 antibody
    • CK3 antibody
    • Cytokeratin-3 antibody
    • K2C3_HUMAN antibody
    • K3 antibody
    • Keratin 3 antibody
    • Keratin antibody
    • Keratin type II cytoskeletal 3 antibody
    • Keratin-3 antibody
    • KRT3 antibody
    • type II cytoskeletal 3 antibody
    • Type-II keratin Kb3 antibody
    see all

Anti-Cytokeratin 3 antibody [AE5] images

  • Overlay histogram showing A431 cells stained with ab77869 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77869, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab77869 staining Cytokeratin 3 (red) in transfected Ptk2 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were co-transfected in quadruplicate with wild-type, mutant, or a 1:1 mixture of wild-type and mutant K12 expression plasmids along with a wild-type K3 expression construct. After 72 hours, cells were fixed with 3% paraformaldehyde for 20 minutes, before being incubated with primary antibody. Nuclei were stained with DAPI (blue).

References for Anti-Cytokeratin 3 antibody [AE5] (ab77869)

This product has been referenced in:
  • Hanson C  et al. Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta Ophthalmol : (2012). Read more (PubMed: 22280565) »
  • Liao H  et al. Development of Allele-Specific Therapeutic siRNA in Meesmann Epithelial Corneal Dystrophy. PLoS One 6:e28582 (2011). ICC/IF ; Human . Read more (PubMed: 22174841) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us and your interest in our products.


As discussed over the phone, the Anti-Cytokeratin 3 antibody [AE5](ab77869) should be suitable for the experiment you are hoping to perform. This particular clone has been u...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Human Cell (tooth)
Specification tooth
Permeabilization Yes - triton x-100
Fixative Formaldehyde
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Abcam user community

Verified customer

Submitted Sep 30 2013

Thank you for your inquiry and your interest in our products.

I searched our catalogue and we currently do not have a cytokeratin 3 antibody that is directly conjugated. We do carry ab77869, which has been tested in FC with rabbit, cow and hu...

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Thank you for contacting Abcam.

I will be happy to help you further in this matter, but I just have a few more questions for you:

1 - Were you staining tissue sections or a monolayer of cultured cells?

2 - If you were stai...

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Thats no problem. If you have any further questions please do not hesitate to contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"