Recombinant Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AE5] to Cytokeratin 3/CK-3
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 3/CK-3 antibody [AE5]
See all Cytokeratin 3/CK-3 primary antibodies -
Description
Mouse monoclonal [AE5] to Cytokeratin 3/CK-3 -
Host species
Mouse -
Specificity
This antibody recognizes the 64kD polypeptide (cytokeratin 3 / CK-3) and 55 kDa polypeptide (cytokeratin 12) of rabbit corneal epithelium. It also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. The clone number has been updated from (2Q1040) to (AE5) both clone numbers name the same antibody clone.
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Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC: Human squamous papilloma of cornea tissue
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General notes
This product has switched from a hybridoma to recombinant production method on 5th January 2022
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AE5 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 0.25 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 0.25 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Cornea specific. -
Involvement in disease
Defects in KRT3 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated as MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3850 Human
- Omim: 148043 Human
- SwissProt: P12035 Human
- Unigene: 680652 Human
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Alternative names
- 65 kDa cytokeratin antibody
- CK-3 antibody
- CK3 antibody
see all
Images
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All lanes : Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260) at 1/5000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector containing a His tag®, whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with Cytokeratin full length expression vector containing a His tag®, whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 64 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
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Immunohistochemical analysis of paraffin-embedded human squamous papilloma of cornea tissue labelling Cytokeratin 3 with ab68260 at 1/500 dilution, followed by a ready-to-use secondary antibody. Cytosolic staining on human squamous papilloma of cornea tissue is observed. Counterstained with Hematoxylin. Positive staining on human squamous papilloma of cornea. The section was incubated with ab68260 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab68260 has been referenced in 27 publications.
- Jaffet J et al. Clinical, histological and immunohistochemistry characteristics of cornea in the sequelae stage of chronic vernal keratoconjunctivitis. Indian J Ophthalmol 70:59-64 (2022). PubMed: 34937208
- Park SK et al. Hyaluronic acid hydrogels crosslinked via blue light-induced thiol-ene reaction for the treatment of rat corneal alkali burn. Regen Ther 20:51-60 (2022). PubMed: 35402662
- Yu Z et al. A human cornea-on-a-chip for the study of epithelial wound healing by extracellular vesicles. iScience 25:104200 (2022). PubMed: 35479406
- Eriksen AZ et al. Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids. STAR Protoc 3:101383 (2022). PubMed: 35664254
- Jumelle C et al. Growth factor-eluting hydrogels for management of corneal defects. Mater Sci Eng C Mater Biol Appl 120:111790 (2021). PubMed: 33545916