Overview

  • Product name
    Anti-Cytokeratin 3+12 antibody [AE5]
  • Description
    Mouse monoclonal [AE5] to Cytokeratin 3+12
  • Specificity
    This antibody recognizes the 64kD polypeptide (cytokeratin 3) and 55 kDa polypeptide (cytokeratin 12) of rabbit corneal epithelium. It also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. The clone number has been updated from (2Q1040) to (AE5) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Cow, Human
  • Immunogen

    Rabbit corneal epithelial keratin.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Clonality
    Monoclonal
  • Clone number
    AE5
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 54 , 65 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50.

Target

  • Relevance
    Cytokeratin 3 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with family member KRT12. Cytokeratin 12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Defects in KRT3 and KRT12 are a cause of Meesmann corneal dystrophy (MCD), an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Symptoms occur in adulthood and include rupture of the corneal microcysts that may lead to photophobia, contact lens intolerance and intermittent diminution of visual acuity. Defects in KRT12 are a cause of juvenile epithelial corneal dystrophy of Meesmann (MCD)
  • Database links
  • Alternative names
    • 65 kDa cytokeratin antibody
    • CK12 antibody
    • CK3 antibody
    • K12 antibody
    • K3 antibody
    • Keratin 12 antibody
    • Keratin 3 antibody
    • Keratin, type I cytoskeletal 12 antibody
    • Keratin, type II cytoskeletal 3 antibody
    • KRT12 antibody
    • KRT3 antibody
    see all

Images

  • Immunofluorescence analysis of rabbit cornea cells, staining Cytokeratin 3+12 with ab68620.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/50 in 3% BSA in PBST (0.5% Tween 20)) for 12 hours at 4°C. An AlexaFluor®594-conjugated goat anti-mouse polyclonal IgG (1/100) was used as the secondary antibody.

    See Abreview

  • Immunofluorescent analysis of human ocular surface squamous cell carcinoma tissue, labeling Cytokeratin 3+12 with ab68260. Samples were fixed in 4% paraformaldehyde and blocked wuth 4% BSA with 0.1% Triton X-100. Incubation with ab68260 (diluted 1/50) overnight at 4°C. Samples were then incubated with an Alexa Fluor 488-conjugated secondary antibody for 1 hour, and counterstained with DAPI to visualize nuclei. 

References

This product has been referenced in:

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Cornea)
Specification
Cornea
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Aaron Gardner

Verified customer

Submitted Nov 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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