• Product nameAnti-Cytokeratin 3+12 antibody [AE5]
  • Description
    Mouse monoclonal [AE5] to Cytokeratin 3+12
  • SpecificityThis antibody recognizes the 64kD polypeptide (cytokeratin 3) and 55 kDa polypeptide (cytokeratin 12) of rabbit corneal epithelium. It also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. The clone number has been updated from (2Q1040) to (AE5) both clone numbers name the same antibody clone.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Cow, Human
  • Immunogen

    Rabbit corneal epithelial keratin.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityIon Exchange Chromatography
  • ClonalityMonoclonal
  • Clone numberAE5
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab68260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 54 , 65 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50.


  • RelevanceCytokeratin 3 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with family member KRT12. Cytokeratin 12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Defects in KRT3 and KRT12 are a cause of Meesmann corneal dystrophy (MCD), an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Symptoms occur in adulthood and include rupture of the corneal microcysts that may lead to photophobia, contact lens intolerance and intermittent diminution of visual acuity. Defects in KRT12 are a cause of juvenile epithelial corneal dystrophy of Meesmann (MCD)
  • Database links
  • Alternative names
    • 65 kDa cytokeratin antibody
    • CK12 antibody
    • CK3 antibody
    • K12 antibody
    • K3 antibody
    • Keratin 12 antibody
    • Keratin 3 antibody
    • Keratin, type I cytoskeletal 12 antibody
    • Keratin, type II cytoskeletal 3 antibody
    • KRT12 antibody
    • KRT3 antibody
    see all

Anti-Cytokeratin 3+12 antibody [AE5] images

  • Immunofluorescence analysis of rabbit cornea cells, staining Cytokeratin 3+12 with ab68620.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/50 in 3% BSA in PBST (0.5% Tween 20)) for 12 hours at 4°C. An AlexaFluor®594-conjugated goat anti-mouse polyclonal IgG (1/100) was used as the secondary antibody.

    See Abreview

  • Immunofluorescent analysis of human ocular surface squamous cell carcinoma tissue, labeling Cytokeratin 3+12 with ab68260. Samples were fixed in 4% paraformaldehyde and blocked wuth 4% BSA with 0.1% Triton X-100. Incubation with ab68260 (diluted 1/50) overnight at 4°C. Samples were then incubated with an Alexa Fluor 488-conjugated secondary antibody for 1 hour, and counterstained with DAPI to visualize nuclei. 

References for Anti-Cytokeratin 3+12 antibody [AE5] (ab68260)

This product has been referenced in:
  • Ouyang H  et al. WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis. Nature 511:358-61 (2014). Read more (PubMed: 25030175) »
  • Li J  et al. S100A expression in normal corneal-limbal epithelial cells and ocular surface squamous cell carcinoma tissue. Mol Vis 17:2263-71 (2011). IHC (PFA fixed) ; Human . Read more (PubMed: 21897749) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rabbit Cell (Cornea)
Specification Cornea
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Dr. Aaron Gardner

Verified customer

Submitted Nov 20 2012