• Product nameAnti-Cytokeratin 4 antibody [6B10]
    See all Cytokeratin 4 primary antibodies
  • Description
    Mouse monoclonal [6B10] to Cytokeratin 4
  • SpecificityReacts exclusively with cytokeratin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands are also positive. Normally keratin 4 is not present in the layers of the epidermis, but should be detectable in glandular tissue of the skin (sweat glands). Skin epidermis contains mainly cytokeratins 14 and 19 (in the basal layer) and cytokeratin 1 and 10 in the cornifying layers. Cytokeratin 4 has a molecular weight of approximately 59 kDa.
  • Tested applicationsSuitable for: ICC, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Cat, Dog, Human, Zebrafish
  • Immunogen

    Cytokeratin preparation extracted from human esophagus.



Our Abpromise guarantee covers the use of ab9004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB 1/100 - 1/1000. Predicted molecular weight: 57 kDa.
Flow Cyt 1/25 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Tissue specificityDetected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
  • Involvement in diseaseDefects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • FormLocalisation: Intermediate filament (Cytoskeleton).
  • Alternative names
    • CK 4 antibody
    • CK-4 antibody
    • CK4 antibody
    • CYK4 antibody
    • Cytokeratin 4 antibody
    • Cytokeratin-4 antibody
    • Cytokeratin4 antibody
    • FLJ31692 antibody
    • K2C4_HUMAN antibody
    • K4 antibody
    • Keratin 4 antibody
    • Keratin antibody
    • Keratin type II cytoskeletal 4 antibody
    • Keratin-4 antibody
    • Keratin4 antibody
    • KRT 4 antibody
    • Krt4 antibody
    • type II cytoskeletal 4 antibody
    • Type-II keratin Kb4 antibody
    see all

Anti-Cytokeratin 4 antibody [6B10] images

  • ab9004 at a 1/100 dilution staining Cytokeratin 4 in human leukoplakia lesions by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Top image: Normal tissue.
    Middle image: Tumor tissue.
    Bottom image: Leukoplakia non-progressing.

    Paraffin sections were deparaffinized, re-hydrated, subjected to antigen retrieval by microwave boiling for 10 minutes in 10 mM TRIS pH 9.0, 1 mM EDTA, and pre-incubated for 15 minutes with 2% normal rabbit serum. The second step was performed with a biotinylated rabbit anti-mouse at a 1/500 dilution and in the final step horseradish peroxidase labeled streptavidin–biotincomplex was applied. The staining was developed with diaminobenzidine and H2O2 as chromogen. The sections were counterstained with haematoxylin and coverslipped with Kaiser’s glycerin.

References for Anti-Cytokeratin 4 antibody [6B10] (ab9004)

This product has been referenced in:
  • Badylak SF  et al. Esophageal preservation in five male patients after endoscopic inner-layer circumferential resection in the setting of superficial cancer: a regenerative medicine approach with a biologic scaffold. Tissue Eng Part A 17:1643-50 (2011). IHC-P ; Human . Read more (PubMed: 21306292) »
  • Schaaij-Visser TB  et al. Evaluation of cornulin, keratin 4, keratin 13 expression and grade of dysplasia for predicting malignant progression of oral leukoplakia. Oral Oncol 46:123-7 (2010). IHC-P ; Human . Read more (PubMed: 20036603) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us about this issue.

You should detect cytokeratin 4 at about 59kDa, assuming your samples are reduced and denatured. Can you please tell me what your samples are, and how they are prepared? Could you also send a few...

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Thank you for contacting Abcam regarding ab9004.

I can confirm that [6B10] in the product name refers to the clone number and is not at all indicative of any modifications to cytokeratin 4.

I hope this information is helpful...

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I apologize for the delay.

Vimentin is a mesenchymal cell marker, ab8978 is tested in Zebrafish. It might be suitable for your purpose.

ab9004, ab9021, ab9217 are anti Cytokeratin 4,7 and 18. Against epithelial cell marker the antibod...

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